Abundance and community structures of heterotrophic and oligotrophic bacteria from the Sierra Leone Abbyssal Plain
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
1992
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| _version_ | 1867168555713167360 |
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| author | Rüger, Hans-Jürgen Tan, Tjhing Lok |
| author_facet | Rüger, Hans-Jürgen Tan, Tjhing Lok |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Colony counts on high and low-nutrient agar media incubated at 2 and 20 °C, Acridine Orange Direct Counts and biomasses are reported for sediments of the Sierra Leone Abyssal Plain. All isolates from low-nutrient agars also grew in nutrient-rich seawater broth (100 % SWB). However, a greater proportion of the 2 °C than of the 20 °C isolates grew in 2.5% SWB, containing 125 mg/l peptone and 25 mg/l yeast extract. Only 14 strains or 12.7% of the 2 °C isolates, but none of the 20 °C isolates, grew in 0.25 % SWB. Psychrophilic bacteria with maximum growth temperatures below 12 °C, isolated at 2 °C, were predominant among the cultivable bacteria from the surface layer. They required seawater for growth and belonged mainly to the Gram-negative genera Alteromonas and Vibrio. In contrast to the earlier view that psychrophily is connected with the Gram-negative cell type, it was found that cold-adapted bacteria of the Gram-positive genus Bacillus predominated in the 4 to 6 cm layer. The 20 °C isolates, however, were mostly Gram-positive, mesophilic, not dependent on seawater for growth, not able to utilize organic substrates at 4 °C, and belonged mainly to the genus Bacillus and to the Gram-positive cocci. The majority of the mesophilic bacilli most likely evolved from dormant spores, but not from actively metabolizing cells. It can be concluded that only the strains isolated at 2 °C can be regarded as indigenous to the deep-sea. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_744000 |
| institution | PANGAEA |
| language | en |
| publishDate | 1992 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Abundance and community structures of heterotrophic and oligotrophic bacteria from the Sierra Leone Abbyssal Plain Rüger, Hans-Jürgen Tan, Tjhing Lok 371; 373; 375; 376; 377; 378; 381; 383; 386; 388; Atlantic Ocean; BC; Box corer; CT; GEOTROPEX 83, NOAMP I; Giant box corer; GIK16421-2; GIK16422-2; GIK16424-1; GIK16425-1; GIK16426-3; GIK16427-1; GIK16430-2; GIK16432-1; GIK16435-1; GIK16437-3; GKG; Gravity corer (Kiel type); M65; M65-track; Meteor (1964); SL; Underway cruise track measurements; van Veen Grab; VGRAB Colony counts on high and low-nutrient agar media incubated at 2 and 20 °C, Acridine Orange Direct Counts and biomasses are reported for sediments of the Sierra Leone Abyssal Plain. All isolates from low-nutrient agars also grew in nutrient-rich seawater broth (100 % SWB). However, a greater proportion of the 2 °C than of the 20 °C isolates grew in 2.5% SWB, containing 125 mg/l peptone and 25 mg/l yeast extract. Only 14 strains or 12.7% of the 2 °C isolates, but none of the 20 °C isolates, grew in 0.25 % SWB. Psychrophilic bacteria with maximum growth temperatures below 12 °C, isolated at 2 °C, were predominant among the cultivable bacteria from the surface layer. They required seawater for growth and belonged mainly to the Gram-negative genera Alteromonas and Vibrio. In contrast to the earlier view that psychrophily is connected with the Gram-negative cell type, it was found that cold-adapted bacteria of the Gram-positive genus Bacillus predominated in the 4 to 6 cm layer. The 20 °C isolates, however, were mostly Gram-positive, mesophilic, not dependent on seawater for growth, not able to utilize organic substrates at 4 °C, and belonged mainly to the genus Bacillus and to the Gram-positive cocci. The majority of the mesophilic bacilli most likely evolved from dormant spores, but not from actively metabolizing cells. It can be concluded that only the strains isolated at 2 °C can be regarded as indigenous to the deep-sea. |
| title | Abundance and community structures of heterotrophic and oligotrophic bacteria from the Sierra Leone Abbyssal Plain |
| topic | 371; 373; 375; 376; 377; 378; 381; 383; 386; 388; Atlantic Ocean; BC; Box corer; CT; GEOTROPEX 83, NOAMP I; Giant box corer; GIK16421-2; GIK16422-2; GIK16424-1; GIK16425-1; GIK16426-3; GIK16427-1; GIK16430-2; GIK16432-1; GIK16435-1; GIK16437-3; GKG; Gravity corer (Kiel type); M65; M65-track; Meteor (1964); SL; Underway cruise track measurements; van Veen Grab; VGRAB |
| url | https://doi.org/10.1594/PANGAEA.744000 |