Turbidity measurements in 96-wells microplates to determine the densities of copiotrophic and oligotrophic bacteria with the Most-Probable-Number-method during POLARSTERN cruise ANT-XI/2
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
1996
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| _version_ | 1867167664245309440 |
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| author | Tan, Tjhing Lok Reinke, Manfred Rüger, Hans-Jürgen |
| author_facet | Tan, Tjhing Lok Reinke, Manfred Rüger, Hans-Jürgen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | A new microtiter-plate dilution method was applied during the expedition ANTARKTIS-XI/2 with RV Polarstern to determine the distribution of copiotrophic and oligotrophic bacteria in the water columns at polar fronts. Twofold serial dilutions were performed with an eight-channel Electrapette in 96-wells plates by mixing 150 µl of seawater with 150 µl of copiotrophic or olitrophic Trypticase-Broth, three times per well. After incubation of about 6 month at 2 °C, turbidities were measured with an eight-channel photometer at 405 nm and combinations of positive test results for three consecutive dilutions chosen and compared with a Most Probable Number table, calculated for 8 replicates and twofold serial dilutions. Densities of 12 to 661 cells/ml for copiotrophs, and 1 to 39 cells/ml for oligotrophs were found. Colony Forming Units on copiotrophic Trypticase-Agar were between 6 and 847 cells/ml, which is in the same range as determined with the MPN method. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_745108 |
| institution | PANGAEA |
| language | en |
| publishDate | 1996 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Turbidity measurements in 96-wells microplates to determine the densities of copiotrophic and oligotrophic bacteria with the Most-Probable-Number-method during POLARSTERN cruise ANT-XI/2 Tan, Tjhing Lok Reinke, Manfred Rüger, Hans-Jürgen ANT-XI/2; MULT; Multiple investigations; Polarstern; PS2489; PS2491; PS2495; PS2496; PS2497; PS2499; PS2500; PS2501; PS2502; PS2510; PS2512; PS28; PS28/256; PS28/264; PS28/293; PS28/298; PS28/301; PS28/314; PS28/316; PS28/329; PS28/334; PS28/339; PS28/358; PS28/367 A new microtiter-plate dilution method was applied during the expedition ANTARKTIS-XI/2 with RV Polarstern to determine the distribution of copiotrophic and oligotrophic bacteria in the water columns at polar fronts. Twofold serial dilutions were performed with an eight-channel Electrapette in 96-wells plates by mixing 150 µl of seawater with 150 µl of copiotrophic or olitrophic Trypticase-Broth, three times per well. After incubation of about 6 month at 2 °C, turbidities were measured with an eight-channel photometer at 405 nm and combinations of positive test results for three consecutive dilutions chosen and compared with a Most Probable Number table, calculated for 8 replicates and twofold serial dilutions. Densities of 12 to 661 cells/ml for copiotrophs, and 1 to 39 cells/ml for oligotrophs were found. Colony Forming Units on copiotrophic Trypticase-Agar were between 6 and 847 cells/ml, which is in the same range as determined with the MPN method. |
| title | Turbidity measurements in 96-wells microplates to determine the densities of copiotrophic and oligotrophic bacteria with the Most-Probable-Number-method during POLARSTERN cruise ANT-XI/2 |
| topic | ANT-XI/2; MULT; Multiple investigations; Polarstern; PS2489; PS2491; PS2495; PS2496; PS2497; PS2499; PS2500; PS2501; PS2502; PS2510; PS2512; PS28; PS28/256; PS28/264; PS28/293; PS28/298; PS28/301; PS28/314; PS28/316; PS28/329; PS28/334; PS28/339; PS28/358; PS28/367 |
| url | https://doi.org/10.1594/PANGAEA.745108 |