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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2009
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.754885 |
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| _version_ | 1867170459180597248 |
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| author | Mertens, Kenneth Neil Verhoeven, Koen Verleye, Thomas Louwye, Stephen Amorim, Ana Ribeiro, Sofia Deaf, Amr S Harding, Ian C De Schepper, Stijn González, Catalina Kodrans-Nsiah, Monika de Vernal, Anne Henry, Maryse Radi, Taoufik Dybkjaer, Karen Poulsen, Niels E Feist-Burkhardt, Susanne Chitolie, Jonah Heilmann-Clausen, Claus Londeix, Laurent Turon, Jean-Louis Marret, Fabienne Matthiessen, Jens McCarthy, Francine M G Prasad, Vandana Pospelova, Vera Hughes, Jane E Kyffin Riding, James B Rochon, André Sangiorgi, Francesca Welters, Natasja Sinclair, Natalie Thun, Christian Soliman, Ali Van Nieuwenhove, Nicolas Vink, Annemiek Young, Martin |
| author_facet | Mertens, Kenneth Neil Verhoeven, Koen Verleye, Thomas Louwye, Stephen Amorim, Ana Ribeiro, Sofia Deaf, Amr S Harding, Ian C De Schepper, Stijn González, Catalina Kodrans-Nsiah, Monika de Vernal, Anne Henry, Maryse Radi, Taoufik Dybkjaer, Karen Poulsen, Niels E Feist-Burkhardt, Susanne Chitolie, Jonah Heilmann-Clausen, Claus Londeix, Laurent Turon, Jean-Louis Marret, Fabienne Matthiessen, Jens McCarthy, Francine M G Prasad, Vandana Pospelova, Vera Hughes, Jane E Kyffin Riding, James B Rochon, André Sangiorgi, Francesca Welters, Natasja Sinclair, Natalie Thun, Christian Soliman, Ali Van Nieuwenhove, Nicolas Vink, Annemiek Young, Martin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_754885 |
| institution | PANGAEA |
| language | en |
| publishDate | 2009 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Absolute abundances of dinoflagellate cysts in surface sediment samples from four sites: Lycopodium marker-grain method Mertens, Kenneth Neil Verhoeven, Koen Verleye, Thomas Louwye, Stephen Amorim, Ana Ribeiro, Sofia Deaf, Amr S Harding, Ian C De Schepper, Stijn González, Catalina Kodrans-Nsiah, Monika de Vernal, Anne Henry, Maryse Radi, Taoufik Dybkjaer, Karen Poulsen, Niels E Feist-Burkhardt, Susanne Chitolie, Jonah Heilmann-Clausen, Claus Londeix, Laurent Turon, Jean-Louis Marret, Fabienne Matthiessen, Jens McCarthy, Francine M G Prasad, Vandana Pospelova, Vera Hughes, Jane E Kyffin Riding, James B Rochon, André Sangiorgi, Francesca Welters, Natasja Sinclair, Natalie Thun, Christian Soliman, Ali Van Nieuwenhove, Nicolas Vink, Annemiek Young, Martin Benguela; Celtic_Sea; Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; MARUM; MUC; MultiCorer; North_Sea; North Sea; NW_Africa Absolute abundances (concentrations) of dinoflagellate cysts are often determined through the addition of Lycopodium clavatum marker-grains as a spike to a sample before palynological processing. An inter-laboratory calibration exercise was set up in order to test the comparability of results obtained in different laboratories, each using its own preparation method. Each of the 23 laboratories received the same amount of homogenized splits of four Quaternary sediment samples. The samples originate from different localities and consisted of a variety of lithologies. Dinoflagellate cysts were extracted and counted, and relative and absolute abundances were calculated. The relative abundances proved to be fairly reproducible, notwithstanding a need for taxonomic calibration. By contrast, excessive loss of Lycopodium spores during sample preparation resulted in non-reproducibility of absolute abundances. Use of oxidation, KOH, warm acids, acetolysis, mesh sizes larger than 15 µm and long ultrasonication (> 1 min) must be avoided to determine reproducible absolute abundances. The results of this work therefore indicate that the dinoflagellate cyst worker should make a choice between using the proposed standard method which circumvents critical steps, adding Lycopodium tablets at the end of the preparation and using an alternative method. |
| title | Absolute abundances of dinoflagellate cysts in surface sediment samples from four sites: Lycopodium marker-grain method |
| topic | Benguela; Celtic_Sea; Center for Marine Environmental Sciences; GeoB; Geosciences, University of Bremen; MARUM; MUC; MultiCorer; North_Sea; North Sea; NW_Africa |
| url | https://doi.org/10.1594/PANGAEA.754885 |