Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2010
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| _version_ | 1867169980256092160 |
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| author | Wu, YaPing Gao, Kunshan Riebesell, Ulf |
| author_facet | Wu, YaPing Gao, Kunshan Riebesell, Ulf |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_758214 |
| institution | PANGAEA |
| language | en |
| publishDate | 2010 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010 Wu, YaPing Gao, Kunshan Riebesell, Ulf Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Carbon dioxide, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Clark type oxygen electrode (5300A, YSI); Comment; DIC analyzer (AS-C3, Apollo Scitech); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Infrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000; Laboratory experiment; Laboratory strains; Light saturation point; Light saturation point, standard deviation; Liquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer); Maximum potential photosynthesis; Maximum potential photosynthesis, standard deviation; Measured; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; pH meter (Benchtop pH510, OAKTON); Photochemical efficiency; Photochemical efficiency, standard deviation; Photosynthetic carbon fixation rate; Photosynthetic carbon fixation rate, standard deviation; Phytoplankton; Primary production/Photosynthesis; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; see reference(s); Single species; Spectrophotometry; Temperature, water CO2/pH perturbation experiments were carried out under two different pCO2 levels (39.3 and 101.3 Pa) to evaluate effects of CO2-induced ocean acidification on the marine diatom Phaeodactylum tricornutum. After acclimation (>20 generations) to ambient and elevated CO2 conditions (with corresponding pH values of 8.15 and 7.80, respectively), growth and photosynthetic carbon fixation rates of high CO2 grown cells were enhanced by 5% and 12%, respectively, and dark respiration stimulated by 34% compared to cells grown at ambient CO2. The half saturation constant (Km) for carbon fixation (dissolved inorganic carbon, DIC) increased by 20% under the low pH and high CO2 condition, reflecting a decreased affinity for HCO3- or/and CO2 and down-regulated carbon concentrating mechanism (CCM). In the high CO2 grown cells, the electron transport rate from photosystem II (PSII) was photoinhibited to a greater extent at high levels of photosynthetically active radiation, while non-photochemical quenching was reduced compared to low CO2 grown cells. This was probably due to the down-regulation of CCM, which serves as a sink for excessive energy. The balance between these positive and negative effects on diatom productivity will be a key factor in determining the net effect of rising atmospheric CO2 on ocean primary production. |
| title | Seawater carbonate chemistry and diatom Phaeodactylum tricornutum (CCMA 106) biological processes during experiments, 2010 |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Carbon dioxide, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Clark type oxygen electrode (5300A, YSI); Comment; DIC analyzer (AS-C3, Apollo Scitech); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Infrared CO2/H2O gas analyzer, non-dispersive, LI-COR type LI-7000; Laboratory experiment; Laboratory strains; Light saturation point; Light saturation point, standard deviation; Liquid scintillation counter (Tri-Carb 2800TR, Perkin-Elmer); Maximum potential photosynthesis; Maximum potential photosynthesis, standard deviation; Measured; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; pH meter (Benchtop pH510, OAKTON); Photochemical efficiency; Photochemical efficiency, standard deviation; Photosynthetic carbon fixation rate; Photosynthetic carbon fixation rate, standard deviation; Phytoplankton; Primary production/Photosynthesis; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; see reference(s); Single species; Spectrophotometry; Temperature, water |
| url | https://doi.org/10.1594/PANGAEA.758214 |