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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2011
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| Online Access: | https://doi.org/10.1594/PANGAEA.770091 |
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| _version_ | 1867171779345121280 |
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| author | Wong, Kevin K W Lane, Ackley Charles Leung, Priscilla TY Thlyagarajan, V |
| author_facet | Wong, Kevin K W Lane, Ackley Charles Leung, Priscilla TY Thlyagarajan, V |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The majority of benthic marine invertebrates have a complex life cycle, during which the pelagic larvae select a suitable substrate, attach to it, and then metamorphose into benthic adults. Anthropogenic ocean acidification (OA) is postulated to affect larval metamorphic success through an altered protein expression pattern (proteome structure) and post-translational modifications. To test this hypothesis, larvae of an economically and ecologically important barnacle species Balanus amphitrite, were cultured from nauplius to the cyprid stage in the present (control) and in the projected elevated concentrations of CO2 for the year 2100 (the OA treatment). Cyprid response to OA was analyzed at the total proteome level as well as two protein post-translational modification (phosphorylation and glycosylation) levels using a 2-DE based proteomic approach. The cyprid proteome showed OA-driven changes. Proteins that were differentially up or down regulated by OA come from three major groups, namely those related to energy-metabolism, respiration, and molecular chaperones, illustrating a potential strategy that the barnacle larvae may employ to tolerate OA stress. The differentially expressed proteins were tentatively identified as OA-responsive, effectively creating unique protein expression signatures for OA scenario of 2100. This study showed the promise of using a sentinel and non-model species to examine the impact of OA at the proteome level. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_770091 |
| institution | PANGAEA |
| language | en |
| publishDate | 2011 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and protein sports of barnicle Balanus amphitrite during experiments, 2011 Wong, Kevin K W Lane, Ackley Charles Leung, Priscilla TY Thlyagarajan, V Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Arthropoda; Balanus amphitrite; Bicarbonate; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard error; Carbon dioxide, total; Coast and continental shelf; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Glycoprotein spots; Identification; Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Pelagos; pH, standard error; pH, total scale; pH meter (Metrohm electrodes); Phosphoprotein spots; Protein spots, total; Salinity; Salinity, standard error; Sample ID; Single species; Temperature, water; Temperature, water, standard error; Tropical; Zooplankton The majority of benthic marine invertebrates have a complex life cycle, during which the pelagic larvae select a suitable substrate, attach to it, and then metamorphose into benthic adults. Anthropogenic ocean acidification (OA) is postulated to affect larval metamorphic success through an altered protein expression pattern (proteome structure) and post-translational modifications. To test this hypothesis, larvae of an economically and ecologically important barnacle species Balanus amphitrite, were cultured from nauplius to the cyprid stage in the present (control) and in the projected elevated concentrations of CO2 for the year 2100 (the OA treatment). Cyprid response to OA was analyzed at the total proteome level as well as two protein post-translational modification (phosphorylation and glycosylation) levels using a 2-DE based proteomic approach. The cyprid proteome showed OA-driven changes. Proteins that were differentially up or down regulated by OA come from three major groups, namely those related to energy-metabolism, respiration, and molecular chaperones, illustrating a potential strategy that the barnacle larvae may employ to tolerate OA stress. The differentially expressed proteins were tentatively identified as OA-responsive, effectively creating unique protein expression signatures for OA scenario of 2100. This study showed the promise of using a sentinel and non-model species to examine the impact of OA at the proteome level. |
| title | Seawater carbonate chemistry and protein sports of barnicle Balanus amphitrite during experiments, 2011 |
| topic | Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Arthropoda; Balanus amphitrite; Bicarbonate; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard error; Carbon dioxide, total; Coast and continental shelf; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Glycoprotein spots; Identification; Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Pelagos; pH, standard error; pH, total scale; pH meter (Metrohm electrodes); Phosphoprotein spots; Protein spots, total; Salinity; Salinity, standard error; Sample ID; Single species; Temperature, water; Temperature, water, standard error; Tropical; Zooplankton |
| url | https://doi.org/10.1594/PANGAEA.770091 |