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| Main Authors: | , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2011
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.770491 |
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| _version_ | 1867168100673126400 |
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| author | Witt, Verena Wild, Christian Anthony, Kenneth R N Diaz-Pulido, Guillermo Uthicke, Sven |
| author_facet | Witt, Verena Wild, Christian Anthony, Kenneth R N Diaz-Pulido, Guillermo Uthicke, Sven |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Rising anthropogenic CO2 emissions acidify the oceans, and cause changes to seawater carbon chemistry. Bacterial biofilm communities reflect environmental disturbances and may rapidly respond to ocean acidification. This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre-industrial (~300 ppm), B) present-day (~400 ppm), C) mid century (~560 ppm) and D) late century (~1140 ppm). Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2-correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. Elevated pCO2 (C + D) shifted biofilm algal communities and significantly increased C and N contents, yet O2 fluxes, measured using in light and dark incubations, remained unchanged. Our findings suggest that bacterial biofilm communities rapidly adapt and reorganize in response to high pCO2 to maintain activity such as oxygen production. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_770491 |
| institution | PANGAEA |
| language | en |
| publishDate | 2011 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry, nitrogen concentration and macro community analyse, 2011 Witt, Verena Wild, Christian Anthony, Kenneth R N Diaz-Pulido, Guillermo Uthicke, Sven Algae; Alkalinity, total; Alkalinity, total, standard error; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthos; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbon, inorganic, total; Carbon, inorganic, total, standard deviation; Carbon, organic, total; Carbon, organic, total, standard deviation; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Community composition and diversity; Diatoms; Element analyser, THERMO NA 2500; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Nitrogen, inorganic; Nitrogen, total; Nitrogen, total, standard deviation; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; pH meter (HQ10-HQ20 Meters, Hach, USA); Potentiometric open-cell titration; Replicates; Rocky-shore community; Salinity; South Pacific; Temperate; Temperature, water; Temperature sensor, HOBO, Onset-UA-001; TOC analyzer (Shimadzu) Rising anthropogenic CO2 emissions acidify the oceans, and cause changes to seawater carbon chemistry. Bacterial biofilm communities reflect environmental disturbances and may rapidly respond to ocean acidification. This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre-industrial (~300 ppm), B) present-day (~400 ppm), C) mid century (~560 ppm) and D) late century (~1140 ppm). Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2-correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. Elevated pCO2 (C + D) shifted biofilm algal communities and significantly increased C and N contents, yet O2 fluxes, measured using in light and dark incubations, remained unchanged. Our findings suggest that bacterial biofilm communities rapidly adapt and reorganize in response to high pCO2 to maintain activity such as oxygen production. |
| title | Seawater carbonate chemistry, nitrogen concentration and macro community analyse, 2011 |
| topic | Algae; Alkalinity, total; Alkalinity, total, standard error; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthos; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbon, inorganic, total; Carbon, inorganic, total, standard deviation; Carbon, organic, total; Carbon, organic, total, standard deviation; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Community composition and diversity; Diatoms; Element analyser, THERMO NA 2500; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Nitrogen, inorganic; Nitrogen, total; Nitrogen, total, standard deviation; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; pH meter (HQ10-HQ20 Meters, Hach, USA); Potentiometric open-cell titration; Replicates; Rocky-shore community; Salinity; South Pacific; Temperate; Temperature, water; Temperature sensor, HOBO, Onset-UA-001; TOC analyzer (Shimadzu) |
| url | https://doi.org/10.1594/PANGAEA.770491 |