Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater
Fuente:
PANGAEA
Salvato in:
| Autori principali: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Natura: | Dataset Open Access |
| Lingua: | en |
| Pubblicazione: |
PANGAEA
2012
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1867169986203615232 |
|---|---|
| author | Ferguson, Andrew Layton, Alice Mailloux, Brian Culligan, Patricia Williams, Daniel Smartt, Abby Sayler, Gary Feighery, John McKay, Larry Knappett, Peter Alexandrova, Ekaterina Arbit, Talia Emch, Michael Escamilla, Veronica Ahmed, Kazi Matin Alam, Md Jahangir Streatfield, P Kim Yunus, Mohammad van Geen, Alexander |
| author_facet | Ferguson, Andrew Layton, Alice Mailloux, Brian Culligan, Patricia Williams, Daniel Smartt, Abby Sayler, Gary Feighery, John McKay, Larry Knappett, Peter Alexandrova, Ekaterina Arbit, Talia Emch, Michael Escamilla, Veronica Ahmed, Kazi Matin Alam, Md Jahangir Streatfield, P Kim Yunus, Mohammad van Geen, Alexander |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides (HuBacteroides)) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens, rotavirus and all pathogens combined. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_774444 |
| institution | PANGAEA |
| language | en |
| publishDate | 2012 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater Ferguson, Andrew Layton, Alice Mailloux, Brian Culligan, Patricia Williams, Daniel Smartt, Abby Sayler, Gary Feighery, John McKay, Larry Knappett, Peter Alexandrova, Ekaterina Arbit, Talia Emch, Michael Escamilla, Veronica Ahmed, Kazi Matin Alam, Md Jahangir Streatfield, P Kim Yunus, Mohammad van Geen, Alexander Arsenic; Bacteroides, gene copies, log; Bacteroides, human associated, gene copies, log; Bangladesh; Bromide; Chloride; Chlorine/Bromine ratio; Coliform bacteria, total, log, most probable number; Coliphage F-RNA, plaque-forming unit, log; Conductivity; DEPTH, sediment/rock; Escherichia coli, gene copies, log; Escherichia coli, log, most probable number; Escherichia coli attaching and effacing, gene copies, log; Feature; Frequency Escherichia coli >1, most probable number; Heat labile enterotoxin, gene copies, log; Identification; Invasion plasmid antigen H, gene copies, log; LATITUDE; LONGITUDE; Nitrogen in ammonium; Nitrogen in nitrate; Nitrogen in nitrite; Outer membrane protein W, gene copies, log; Oxidation reduction (RedOx) potential; pH; Phosphate; Platform; Rotavirus nucleocapsid protein, gene copies, log; Salinity; Shiga toxin 1, gene copies, log; Sulfate; Temperature, water; Tubewell; TWELL Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides (HuBacteroides)) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens, rotavirus and all pathogens combined. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary. |
| title | Comparison of fecal indicators with pathogenic bacteria and rotavirus in rural Bangladesh groundwater |
| topic | Arsenic; Bacteroides, gene copies, log; Bacteroides, human associated, gene copies, log; Bangladesh; Bromide; Chloride; Chlorine/Bromine ratio; Coliform bacteria, total, log, most probable number; Coliphage F-RNA, plaque-forming unit, log; Conductivity; DEPTH, sediment/rock; Escherichia coli, gene copies, log; Escherichia coli, log, most probable number; Escherichia coli attaching and effacing, gene copies, log; Feature; Frequency Escherichia coli >1, most probable number; Heat labile enterotoxin, gene copies, log; Identification; Invasion plasmid antigen H, gene copies, log; LATITUDE; LONGITUDE; Nitrogen in ammonium; Nitrogen in nitrate; Nitrogen in nitrite; Outer membrane protein W, gene copies, log; Oxidation reduction (RedOx) potential; pH; Phosphate; Platform; Rotavirus nucleocapsid protein, gene copies, log; Salinity; Shiga toxin 1, gene copies, log; Sulfate; Temperature, water; Tubewell; TWELL |
| url | https://doi.org/10.1594/PANGAEA.774444 |