Phosphorylation levels of JAK2 after addition of increasing Epo-concentrations: experimental data (Figure 5a)
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| Autores principales: | , , , , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2012
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| _version_ | 1867169042061590528 |
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| author | Schilling, Marcel Maiwald, Thomas Hengl, Stefan Winter, Dominic Kreutz, Clemens Kolch, Walter Lehmann, Wolf D Timmer, Jens Klingmüller, Ursula |
| author_facet | Schilling, Marcel Maiwald, Thomas Hengl, Stefan Winter, Dominic Kreutz, Clemens Kolch, Walter Lehmann, Wolf D Timmer, Jens Klingmüller, Ursula |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The present dataset data contain source data for Figure 5a from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. CFU-E cells were stimulated with the indicated Epo concentrations for 7 min and phosphorylation levels were determined by quantitative immunoblotting. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_775540 |
| institution | PANGAEA |
| language | en |
| publishDate | 2012 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Phosphorylation levels of JAK2 after addition of increasing Epo-concentrations: experimental data (Figure 5a) Schilling, Marcel Maiwald, Thomas Hengl, Stefan Winter, Dominic Kreutz, Clemens Kolch, Walter Lehmann, Wolf D Timmer, Jens Klingmüller, Ursula Amount concentration (molecules in cells), of phosphorylated Janus Activated Kinase 2; Event label; Experiment_pJAK2; Treatment: erythropoietin The present dataset data contain source data for Figure 5a from Schilling et al., 2009. Cell fate decisions are regulated by the coordinated activation of signalling pathways such as the extracellular signal-regulated kinase (ERK) cascade, but contributions of individual kinase isoforms are mostly unknown. The authors combined quantitative data from erythropoietin-induced pathway activation in primary erythroid progenitor (colony-forming unit erythroid stage, CFU-E) cells with mathematical modelling, in order to predict and experimentally confirmed a distributive ERK phosphorylation mechanism in CFU-E cells. The authors found evidences that double-phosphorylated ERK1 attenuates proliferation beyond a certain activation level, whereas activated ERK2 enhances proliferation with saturation kinetics. CFU-E cells were stimulated with the indicated Epo concentrations for 7 min and phosphorylation levels were determined by quantitative immunoblotting. |
| title | Phosphorylation levels of JAK2 after addition of increasing Epo-concentrations: experimental data (Figure 5a) |
| topic | Amount concentration (molecules in cells), of phosphorylated Janus Activated Kinase 2; Event label; Experiment_pJAK2; Treatment: erythropoietin |
| url | https://doi.org/10.1594/PANGAEA.775540 |