Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2012
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| _version_ | 1867172318986371072 |
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| author | Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
| author_facet | Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_779704 |
| institution | PANGAEA |
| language | en |
| publishDate | 2012 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 Nakamura, Masoko Morita, Masaya Kurihara, Haruko Mitarai, Satoshi Acropora digitifera; Acropora digitifera, heat shock factor 1 expression; Acropora digitifera, heat shock factor 1 expression, standard error; Acropora digitifera, heat shock protein 70 expression; Acropora digitifera, heat shock protein 70 expression, standard error; Acropora digitifera, heat shock protein 90 expression; Acropora digitifera, heat shock protein 90 expression, standard error; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; pH meter (Mettler Toledo, USA); Salinity; Salinometer (AS ONE, IS/Mill-E); see reference(s); Single species; Temperate; Temperature, water; Total alkalinity titration analyzer (Kimoto ATT-05, Japan) Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. |
| title | Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012 |
| topic | Acropora digitifera; Acropora digitifera, heat shock factor 1 expression; Acropora digitifera, heat shock factor 1 expression, standard error; Acropora digitifera, heat shock protein 70 expression; Acropora digitifera, heat shock protein 70 expression, standard error; Acropora digitifera, heat shock protein 90 expression; Acropora digitifera, heat shock protein 90 expression, standard error; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; pH meter (Mettler Toledo, USA); Salinity; Salinometer (AS ONE, IS/Mill-E); see reference(s); Single species; Temperate; Temperature, water; Total alkalinity titration analyzer (Kimoto ATT-05, Japan) |
| url | https://doi.org/10.1594/PANGAEA.779704 |