(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity
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| Auteurs principaux: | , , , , , , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2011
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| _version_ | 1867171816362999808 |
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| author | McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
| author_facet | McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_816176 |
| institution | PANGAEA |
| language | en |
| publishDate | 2011 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J 7-ethoxyresorufin-O-deethylase, activity per protein mass; Age, comment; Area/locality; Biological sample; BIOS; Cumberland_Sound; DATE/TIME; Event label; Fluorescence microplate assay; Hudson Bay; Iceland; International Polar Year (2007-2008); IPY; Labrador Sea; MULT; Multiple investigations; Proteins, total; Proteins, total, standard deviation; Sample ID; Sample type; Sex; Species; Species, common name; Standard deviation; W_Hudson_Bay The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. |
| title | (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity |
| topic | 7-ethoxyresorufin-O-deethylase, activity per protein mass; Age, comment; Area/locality; Biological sample; BIOS; Cumberland_Sound; DATE/TIME; Event label; Fluorescence microplate assay; Hudson Bay; Iceland; International Polar Year (2007-2008); IPY; Labrador Sea; MULT; Multiple investigations; Proteins, total; Proteins, total, standard deviation; Sample ID; Sample type; Sex; Species; Species, common name; Standard deviation; W_Hudson_Bay |
| url | https://doi.org/10.1594/PANGAEA.816176 |