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| Main Authors: | , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2012
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| Online Access: | https://doi.org/10.1594/PANGAEA.823757 |
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| _version_ | 1867170034685575168 |
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| author | Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen |
| author_facet | Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_823757 |
| institution | PANGAEA |
| language | en |
| publishDate | 2012 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Analysis of Pacific oyster larval proteome and its response to high-CO2 Dineshram, R Wong, Kevin K W Shu, Xiao Yu, Ziniu Qian, Pei Yuan Thiyagarajan, Vengatesen Alkalinity, total; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Duration, number of days; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Growth/Morphology; Identification; Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, total scale; Protein name; Replicate; Salinity; Shell length; Single species; Species; Spot intensity, relative; Temperature, water; Treatment; Tropical; Zooplankton Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. |
| title | Analysis of Pacific oyster larval proteome and its response to high-CO2 |
| topic | Alkalinity, total; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Duration, number of days; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Growth/Morphology; Identification; Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, total scale; Protein name; Replicate; Salinity; Shell length; Single species; Species; Spot intensity, relative; Temperature, water; Treatment; Tropical; Zooplankton |
| url | https://doi.org/10.1594/PANGAEA.823757 |