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Main Authors: Van de Waal, Dedmer B, John, Uwe, Ziveri, Patrizia, Reichart, Gert-Jan, Hoins, Mirja, Sluijs, Appy, Rost, Björn
Format: Dataset Open Access
Language:en
Published: PANGAEA 2013
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Online Access:https://doi.org/10.1594/PANGAEA.824705
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author Van de Waal, Dedmer B
John, Uwe
Ziveri, Patrizia
Reichart, Gert-Jan
Hoins, Mirja
Sluijs, Appy
Rost, Björn
author_facet Van de Waal, Dedmer B
John, Uwe
Ziveri, Patrizia
Reichart, Gert-Jan
Hoins, Mirja
Sluijs, Appy
Rost, Björn
collection Datos científicos de ciencias marinas y ambientales
contents Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_824705
institution PANGAEA
language en
publishDate 2013
publisher PANGAEA
record_format pangaea
spellingShingle Seawater carbonate chemistry and growth, calcification of Thoracosphaera heimii in a laboratory experiment
Van de Waal, Dedmer B
John, Uwe
Ziveri, Patrizia
Reichart, Gert-Jan
Hoins, Mirja
Sluijs, Appy
Rost, Björn
Abundance per volume; Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, particulate, production per cell; Carbon, organic, particulate, production per cell; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide (water), partial pressure, at equilibrator temperature (wet air); Category; Chromista; Cysts; Fractionation of calcite; Fractionation of organic carbon; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Growth/Morphology; Growth rate; Identification; Incubation duration; Laboratory experiment; Laboratory strains; Mediterranean Sea Acidification in a Changing Climate; MedSeA; Myzozoa; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Pelagos; pH, NBS scale; pH, total scale; Phytoplankton; Potentiometric; Potentiometric titration; Protein name; Replicates; Salinity; Single species; Species; Temperature, water; Thoracosphaera heimii; Treatment; δ18O, calcite; δ18O, dissolved inorganic carbon
Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii.
title Seawater carbonate chemistry and growth, calcification of Thoracosphaera heimii in a laboratory experiment
topic Abundance per volume; Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, particulate, production per cell; Carbon, organic, particulate, production per cell; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide (water), partial pressure, at equilibrator temperature (wet air); Category; Chromista; Cysts; Fractionation of calcite; Fractionation of organic carbon; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Growth/Morphology; Growth rate; Identification; Incubation duration; Laboratory experiment; Laboratory strains; Mediterranean Sea Acidification in a Changing Climate; MedSeA; Myzozoa; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Pelagos; pH, NBS scale; pH, total scale; Phytoplankton; Potentiometric; Potentiometric titration; Protein name; Replicates; Salinity; Single species; Species; Temperature, water; Thoracosphaera heimii; Treatment; δ18O, calcite; δ18O, dissolved inorganic carbon
url https://doi.org/10.1594/PANGAEA.824705