Experiment: Growth rates and percentages of normal versus malformed versus incomplete versus incomplete and malformed coccoliths
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| Natura: | Dataset Open Access |
| Lingua: | en |
| Pubblicazione: |
PANGAEA
2014
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| _version_ | 1867169090983952384 |
|---|---|
| author | Langer, Gerald |
| author_facet | Langer, Gerald |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler was cultured in natural seawater with the addition of either the microtubule-inhibitor colchicine, the actin-inhibitor cytochalasin B, or the photosynthesis inhibitor 3-(3,4 dichlorophenyl)-1,1-dimethyl-urea (DCMU). Additionally, E. huxleyi was cultured at different light intensities and temperatures. Growth rate was monitored, and coccolith morphology analyzed. While every treatment affected growth rate, the percentage of malformed coccoliths increased with colchicine, cytochalasin B, and at higher than optimal temperature. These results represent the first experimental evidence for the role of microtubules and actin microfilaments in coccolith morphogenesis. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_829716 |
| institution | PANGAEA |
| language | en |
| publishDate | 2014 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Experiment: Growth rates and percentages of normal versus malformed versus incomplete versus incomplete and malformed coccoliths Langer, Gerald BIOACID; Biological Impacts of Ocean Acidification; Coccoliths, incomplete; Coccoliths, incomplete, standard deviation; Coccoliths, malformed and incomplete; Coccoliths, malformed and incomplete, standard deviation; Coccoliths, normal; Coccoliths, normal, standard deviation; Growth rate; Growth rate, standard deviation; Malformation rate; Malformation rate, standard deviation; Treatment; Variable The coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler was cultured in natural seawater with the addition of either the microtubule-inhibitor colchicine, the actin-inhibitor cytochalasin B, or the photosynthesis inhibitor 3-(3,4 dichlorophenyl)-1,1-dimethyl-urea (DCMU). Additionally, E. huxleyi was cultured at different light intensities and temperatures. Growth rate was monitored, and coccolith morphology analyzed. While every treatment affected growth rate, the percentage of malformed coccoliths increased with colchicine, cytochalasin B, and at higher than optimal temperature. These results represent the first experimental evidence for the role of microtubules and actin microfilaments in coccolith morphogenesis. |
| title | Experiment: Growth rates and percentages of normal versus malformed versus incomplete versus incomplete and malformed coccoliths |
| topic | BIOACID; Biological Impacts of Ocean Acidification; Coccoliths, incomplete; Coccoliths, incomplete, standard deviation; Coccoliths, malformed and incomplete; Coccoliths, malformed and incomplete, standard deviation; Coccoliths, normal; Coccoliths, normal, standard deviation; Growth rate; Growth rate, standard deviation; Malformation rate; Malformation rate, standard deviation; Treatment; Variable |
| url | https://doi.org/10.1594/PANGAEA.829716 |