Experiment: Metabolic shifts in the Antarctic fish Notothenia rossii in response to rising temperature and PCO2
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2014
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| _version_ | 1867170970948599808 |
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| author | Strobel, Anneli Bennecke, Swaantje Leo, Elettra Mintenbeck, Katja Pörtner, Hans-Otto Mark, Felix Christopher |
| author_facet | Strobel, Anneli Bennecke, Swaantje Leo, Elettra Mintenbeck, Katja Pörtner, Hans-Otto Mark, Felix Christopher |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Introduction Ongoing ocean warming and acidification increasingly affect marine ecosystems, in particular around the Antarctic Peninsula. Yet little is known about the capability of Antarctic notothenioid fish to cope with rising temperature in acidifying seawater. While the whole animal level is expected to be more sensitive towards hypercapnia and temperature, the basis of thermal tolerance is set at the cellular level, with a putative key role for mitochondria. This study therefore investigates the physiological responses of the Antarctic Notothenia rossii after long-term acclimation to increased temperatures (7°C) and elevated PCO2 (0.2 kPa CO2) at different levels of physiological organisation. Results For an integrated picture, we analysed the acclimation capacities of N. rossii by measuring routine metabolic rate (RMR), mitochondrial capacities (state III respiration) as well as intra- and extracellular acid-base status during acute thermal challenges and after long-term acclimation to changing temperature and hypercapnia. RMR was partially compensated during warm- acclimation (decreased below the rate observed after acute warming), while elevated PCO2 had no effect on cold or warm acclimated RMR. Mitochondrial state III respiration was unaffected by temperature acclimation but depressed in cold and warm hypercapnia-acclimated fish. In both cold- and warm-exposed N. rossii, hypercapnia acclimation resulted in a shift of extracellular pH (pHe) towards more alkaline values. A similar overcompensation was visible in muscle intracellular pH (pHi). pHi in liver displayed a slight acidosis after warm normo- or hypercapnia acclimation, nevertheless, long-term exposure to higher PCO2 was compensated for by intracellular bicarbonate accumulation. Conclusion The partial warm compensation in whole animal metabolic rate indicates beginning limitations in tissue oxygen supply after warm-acclimation of N. rossii. Compensatory mechanisms of the reduced mitochondrial capacities under chronic hypercapnia may include a new metabolic equilibrium to meet the elevated energy demand for acid-base regulation. New set points of acid-base regulation under hypercapnia, visible at the systemic and intracellular level, indicate that N. rossii can at least in part acclimate to ocean warming and acidification. It remains open whether the reduced capacities of mitochondrial energy metabolism are adaptive or would impair population fitness over longer timescales under chronically elevated temperature and PCO2. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_829830 |
| institution | PANGAEA |
| language | en |
| publishDate | 2014 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Experiment: Metabolic shifts in the Antarctic fish Notothenia rossii in response to rising temperature and PCO2 Strobel, Anneli Bennecke, Swaantje Leo, Elettra Mintenbeck, Katja Pörtner, Hans-Otto Mark, Felix Christopher Carbon dioxide; Carlini/Jubany Station; Condition factor; Gender; Haematocrit; Hepatosomatic index; Jubany_Dallmann; Lactate; Length, standard; Length, total; MULT; Multiple investigations; Osmotic concentration; pH, extracellular; pH, intracellular; PotterCove; Potter Cove, King George Island, Antarctic Peninsula; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Respiration rate, oxygen; Species; SPP1158; Treatment: temperature; Wet mass Introduction Ongoing ocean warming and acidification increasingly affect marine ecosystems, in particular around the Antarctic Peninsula. Yet little is known about the capability of Antarctic notothenioid fish to cope with rising temperature in acidifying seawater. While the whole animal level is expected to be more sensitive towards hypercapnia and temperature, the basis of thermal tolerance is set at the cellular level, with a putative key role for mitochondria. This study therefore investigates the physiological responses of the Antarctic Notothenia rossii after long-term acclimation to increased temperatures (7°C) and elevated PCO2 (0.2 kPa CO2) at different levels of physiological organisation. Results For an integrated picture, we analysed the acclimation capacities of N. rossii by measuring routine metabolic rate (RMR), mitochondrial capacities (state III respiration) as well as intra- and extracellular acid-base status during acute thermal challenges and after long-term acclimation to changing temperature and hypercapnia. RMR was partially compensated during warm- acclimation (decreased below the rate observed after acute warming), while elevated PCO2 had no effect on cold or warm acclimated RMR. Mitochondrial state III respiration was unaffected by temperature acclimation but depressed in cold and warm hypercapnia-acclimated fish. In both cold- and warm-exposed N. rossii, hypercapnia acclimation resulted in a shift of extracellular pH (pHe) towards more alkaline values. A similar overcompensation was visible in muscle intracellular pH (pHi). pHi in liver displayed a slight acidosis after warm normo- or hypercapnia acclimation, nevertheless, long-term exposure to higher PCO2 was compensated for by intracellular bicarbonate accumulation. Conclusion The partial warm compensation in whole animal metabolic rate indicates beginning limitations in tissue oxygen supply after warm-acclimation of N. rossii. Compensatory mechanisms of the reduced mitochondrial capacities under chronic hypercapnia may include a new metabolic equilibrium to meet the elevated energy demand for acid-base regulation. New set points of acid-base regulation under hypercapnia, visible at the systemic and intracellular level, indicate that N. rossii can at least in part acclimate to ocean warming and acidification. It remains open whether the reduced capacities of mitochondrial energy metabolism are adaptive or would impair population fitness over longer timescales under chronically elevated temperature and PCO2. |
| title | Experiment: Metabolic shifts in the Antarctic fish Notothenia rossii in response to rising temperature and PCO2 |
| topic | Carbon dioxide; Carlini/Jubany Station; Condition factor; Gender; Haematocrit; Hepatosomatic index; Jubany_Dallmann; Lactate; Length, standard; Length, total; MULT; Multiple investigations; Osmotic concentration; pH, extracellular; pH, intracellular; PotterCove; Potter Cove, King George Island, Antarctic Peninsula; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Respiration rate, oxygen; Species; SPP1158; Treatment: temperature; Wet mass |
| url | https://doi.org/10.1594/PANGAEA.829830 |