Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013
Fuente:
PANGAEA
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Dataset Open Access |
| Sprache: | en |
| Veröffentlicht: |
PANGAEA
2014
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1867171233148174336 |
|---|---|
| author | Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
| author_facet | Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_835180 |
| institution | PANGAEA |
| language | en |
| publishDate | 2014 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 Schmithüsen, Tosia Niehoff, Barbara Auel, Holger Basin Scale Analysis, Synthesis and Integration; Date/Time of event; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic Ocean; Number of individuals; Proteins per individual; Sample ID; Taxon/taxa; TriStar LB 941 Microplate Reader (Berthold Technologies); Uniform resource locator/link to reference For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies). |
| title | Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 |
| topic | Basin Scale Analysis, Synthesis and Integration; Date/Time of event; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic Ocean; Number of individuals; Proteins per individual; Sample ID; Taxon/taxa; TriStar LB 941 Microplate Reader (Berthold Technologies); Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.835180 |