Combined effects of CO2 and light on large and small isolates of the unicellular N2-fixing cyanobacterium Crocosphaera watsonii from the western tropical Atlantic Ocean
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| Natura: | Dataset Open Access |
| Lingua: | en |
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2013
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| author | Garcia, Nathan S Fu, Feixue Breene, Cynthia L Yu, Elizabeth K Bernhardt, Peter W Mulholland, Margaret R Hutchins, David A |
| author_facet | Garcia, Nathan S Fu, Feixue Breene, Cynthia L Yu, Elizabeth K Bernhardt, Peter W Mulholland, Margaret R Hutchins, David A |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We examined the combined effects of light and pCO2 on growth, CO2-fixation and N2-fixation rates by strains of the unicellular marine N2-fixing cyanobacterium Crocosphaera watsonii with small (WH0401) and large (WH0402) cells that were isolated from the western tropical Atlantic Ocean. In low-pCO2-acclimated cultures (190 ppm) of WH0401, growth, CO2-fixation and N2-fixation rates were significantly lower than those in cultures acclimated to higher (present-day 385 ppm, or future 750 ppm) pCO2 treatments. Growth rates were not significantly different, however, in low-pCO2-acclimated cultures of WH0402 in comparison with higher pCO2 treatments. Unlike previous reports for C. watsonii (strain WH8501), N2-fixation rates did not increase further in cultures of WH0401 or WH0402 when acclimated to 750 ppm relative to those maintained at present-day pCO2. Both light and pCO2 had a significant negative effect on gross : net N2-fixation rates in WH0402 and trends were similar in WH0401, implying that retention of fixed N was enhanced under elevated light and pCO2. These data, along with previously reported results, suggest that C. watsonii may have wide-ranging, strain-specific responses to changing light and pCO2, emphasizing the need for examining the effects of global change on a range of isolates within this biogeochemically important genus. In general, however, our data suggest that cellular N retention and CO2-fixation rates of C. watsonii may be positively affected by elevated light and pCO2 within the next 100 years, potentially increasing trophic transfer efficiency of C and N and thereby facilitating uptake of atmospheric carbon by the marine biota. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_835431 |
| institution | PANGAEA |
| language | en |
| publishDate | 2013 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Combined effects of CO2 and light on large and small isolates of the unicellular N2-fixing cyanobacterium Crocosphaera watsonii from the western tropical Atlantic Ocean Garcia, Nathan S Fu, Feixue Breene, Cynthia L Yu, Elizabeth K Bernhardt, Peter W Mulholland, Margaret R Hutchins, David A 15N2-tracer method; Alkalinity, total; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon fixation rate, photosynthetic; Coulometric titration; Crocosphaera watsonii; Cyanobacteria; Diameter; Diameter, standard error; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light; Nitrogen, particulate, accumulation, per cell; Nitrogen, particulate, accumulation, standard deviation; Nitrogen fixation rate; Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio; Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio, standard deviation; Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio; Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio, standard deviation; Nitrogen fixation rate, per cell; Nitrogen fixation rate, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Photosynthetic carbon fixation rate, standard deviation; Photosynthetic carbon fixation rate per cell; Phytoplankton; Potentiometric; Primary production/Photosynthesis; Salinity; Single species; Species; Strain; Temperature, water We examined the combined effects of light and pCO2 on growth, CO2-fixation and N2-fixation rates by strains of the unicellular marine N2-fixing cyanobacterium Crocosphaera watsonii with small (WH0401) and large (WH0402) cells that were isolated from the western tropical Atlantic Ocean. In low-pCO2-acclimated cultures (190 ppm) of WH0401, growth, CO2-fixation and N2-fixation rates were significantly lower than those in cultures acclimated to higher (present-day 385 ppm, or future 750 ppm) pCO2 treatments. Growth rates were not significantly different, however, in low-pCO2-acclimated cultures of WH0402 in comparison with higher pCO2 treatments. Unlike previous reports for C. watsonii (strain WH8501), N2-fixation rates did not increase further in cultures of WH0401 or WH0402 when acclimated to 750 ppm relative to those maintained at present-day pCO2. Both light and pCO2 had a significant negative effect on gross : net N2-fixation rates in WH0402 and trends were similar in WH0401, implying that retention of fixed N was enhanced under elevated light and pCO2. These data, along with previously reported results, suggest that C. watsonii may have wide-ranging, strain-specific responses to changing light and pCO2, emphasizing the need for examining the effects of global change on a range of isolates within this biogeochemically important genus. In general, however, our data suggest that cellular N retention and CO2-fixation rates of C. watsonii may be positively affected by elevated light and pCO2 within the next 100 years, potentially increasing trophic transfer efficiency of C and N and thereby facilitating uptake of atmospheric carbon by the marine biota. |
| title | Combined effects of CO2 and light on large and small isolates of the unicellular N2-fixing cyanobacterium Crocosphaera watsonii from the western tropical Atlantic Ocean |
| topic | 15N2-tracer method; Alkalinity, total; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon fixation rate, photosynthetic; Coulometric titration; Crocosphaera watsonii; Cyanobacteria; Diameter; Diameter, standard error; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light; Nitrogen, particulate, accumulation, per cell; Nitrogen, particulate, accumulation, standard deviation; Nitrogen fixation rate; Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio; Nitrogen fixation rate, gross/nitrogen fixation rate, netto ratio, standard deviation; Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio; Nitrogen fixation rate, gross/particulate nitrogen, netto, accumulation rate ratio, standard deviation; Nitrogen fixation rate, per cell; Nitrogen fixation rate, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Photosynthetic carbon fixation rate, standard deviation; Photosynthetic carbon fixation rate per cell; Phytoplankton; Potentiometric; Primary production/Photosynthesis; Salinity; Single species; Species; Strain; Temperature, water |
| url | https://doi.org/10.1594/PANGAEA.835431 |