Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2014
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| _version_ | 1867170049911947264 |
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| author | Li, Yahe Xu, Juntian Gao, Kunshan |
| author_facet | Li, Yahe Xu, Juntian Gao, Kunshan |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification (OA) due to atmospheric CO2 rise is expected to influence marine primary productivity. In order to investigate the interactive effects of OA and light changes on diatoms, we grew Phaeodactylum tricornutum, under ambient (390 ppmv; LC) and elevated CO2 (1000 ppmv; HC) conditions for 80 generations, and measured its physiological performance under different light levels (60 µmol/m**2/s, LL; 200 µmol/m**2/s, ML; 460 µmol/m**2/s, HL) for another 25 generations. The specific growth rate of the HC-grown cells was higher (about 12-18%) than that of the LC-grown ones, with the highest under the ML level. With increasing light levels, the effective photochemical yield of PSII (Fv'/Fm') decreased, but was enhanced by the elevated CO2, especially under the HL level. The cells acclimated to the HC condition showed a higher recovery rate of their photochemical yield of PSII compared to the LC-grown cells. For the HC-grown cells, dissolved inorganic carbon or CO2 levels for half saturation of photosynthesis (K1/2 DIC or K1/2 CO2) increased by 11, 55 and 32%, under the LL, ML and HL levels, reflecting a light dependent down-regulation of carbon concentrating mechanisms (CCMs). The linkage between higher level of the CCMs down-regulation and higher growth rate at ML under OA supports the theory that the saved energy from CCMs down-regulation adds on to enhance the growth of the diatom. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_837678 |
| institution | PANGAEA |
| language | en |
| publishDate | 2014 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum Li, Yahe Xu, Juntian Gao, Kunshan Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, reciprocal of photosynthetic affinity value; Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation; Carotenoids, standard deviation; Carotenoids per cell; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chlorophyll c, standard deviation; Chlorophyll c per cell; Chromista; Effective photochemical quantum yield; Effective photochemical quantum yield, standard deviation; Effective quantum yield; Effective quantum yield, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light; Non photochemical quenching; Non photochemical quenching, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; Phytoplankton; Potentiometric; Primary production/Photosynthesis; Salinity; Single species; Species; Temperature, water; Time in days; Time in minutes; Treatment Ocean acidification (OA) due to atmospheric CO2 rise is expected to influence marine primary productivity. In order to investigate the interactive effects of OA and light changes on diatoms, we grew Phaeodactylum tricornutum, under ambient (390 ppmv; LC) and elevated CO2 (1000 ppmv; HC) conditions for 80 generations, and measured its physiological performance under different light levels (60 µmol/m**2/s, LL; 200 µmol/m**2/s, ML; 460 µmol/m**2/s, HL) for another 25 generations. The specific growth rate of the HC-grown cells was higher (about 12-18%) than that of the LC-grown ones, with the highest under the ML level. With increasing light levels, the effective photochemical yield of PSII (Fv'/Fm') decreased, but was enhanced by the elevated CO2, especially under the HL level. The cells acclimated to the HC condition showed a higher recovery rate of their photochemical yield of PSII compared to the LC-grown cells. For the HC-grown cells, dissolved inorganic carbon or CO2 levels for half saturation of photosynthesis (K1/2 DIC or K1/2 CO2) increased by 11, 55 and 32%, under the LL, ML and HL levels, reflecting a light dependent down-regulation of carbon concentrating mechanisms (CCMs). The linkage between higher level of the CCMs down-regulation and higher growth rate at ML under OA supports the theory that the saved energy from CCMs down-regulation adds on to enhance the growth of the diatom. |
| title | Light-modulated responses of growth and photosynthetic performance to ocean acidification in the model diatom Phaeodactylum tricornutum |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, reciprocal of photosynthetic affinity value; Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation; Carotenoids, standard deviation; Carotenoids per cell; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chlorophyll c, standard deviation; Chlorophyll c per cell; Chromista; Effective photochemical quantum yield; Effective photochemical quantum yield, standard deviation; Effective quantum yield; Effective quantum yield, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light; Non photochemical quenching; Non photochemical quenching, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; Phytoplankton; Potentiometric; Primary production/Photosynthesis; Salinity; Single species; Species; Temperature, water; Time in days; Time in minutes; Treatment |
| url | https://doi.org/10.1594/PANGAEA.837678 |