Adaptation of a globally important coccolithophore to ocean warming and acidification
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| Sprache: | en |
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2014
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| _version_ | 1867168158634213376 |
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| author | Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
| author_facet | Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_837880 |
| institution | PANGAEA |
| language | en |
| publishDate | 2014 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Adaptation of a globally important coccolithophore to ocean warming and acidification Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D Acid-base regulation; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, extracellular; pH, intracellular; pH, NBS scale; pH, standard error; pH, total scale; pH change; Pocillopora damicornis; Replicate; Salinity; Salinity, standard error; Single species; Species; Temperature, water; Temperature, water, standard error; Time in minutes; Treatment; Tropical Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. |
| title | Adaptation of a globally important coccolithophore to ocean warming and acidification |
| topic | Acid-base regulation; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, extracellular; pH, intracellular; pH, NBS scale; pH, standard error; pH, total scale; pH change; Pocillopora damicornis; Replicate; Salinity; Salinity, standard error; Single species; Species; Temperature, water; Temperature, water, standard error; Time in minutes; Treatment; Tropical |
| url | https://doi.org/10.1594/PANGAEA.837880 |