Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods
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| Autores principales: | , , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2015
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| _version_ | 1867168162756165632 |
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| author | Hu, Marian Y Sucre, Elliott Charmantier-Daures, Mireille Charmantier, Guy Lucassen, Magnus Himmerkus, Nina Melzner, Frank |
| author_facet | Hu, Marian Y Sucre, Elliott Charmantier-Daures, Mireille Charmantier, Guy Lucassen, Magnus Himmerkus, Nina Melzner, Frank |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_842790 |
| institution | PANGAEA |
| language | en |
| publishDate | 2015 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods Hu, Marian Y Sucre, Elliott Charmantier-Daures, Mireille Charmantier, Guy Lucassen, Magnus Himmerkus, Nina Melzner, Frank Adenosine triphosphatase activity; BIOACID; Biological Impacts of Ocean Acidification; Change in Sodium/potassium ATPase alpha subunit expression; Replicates; Sodium/Potassium adenosine triphosphatase activity; Species; Standard deviation; Type The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances. |
| title | Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods |
| topic | Adenosine triphosphatase activity; BIOACID; Biological Impacts of Ocean Acidification; Change in Sodium/potassium ATPase alpha subunit expression; Replicates; Sodium/Potassium adenosine triphosphatase activity; Species; Standard deviation; Type |
| url | https://doi.org/10.1594/PANGAEA.842790 |