Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2
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| Main Authors: | , , , , , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2015
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| _version_ | 1867170985094938624 |
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| author | Wei, Lei Wang, Qing Ning, Xuanxuan Mu, Changkao Wang, Chunlin Cao, Ruiwen Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin |
| author_facet | Wei, Lei Wang, Qing Ning, Xuanxuan Mu, Changkao Wang, Chunlin Cao, Ruiwen Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_846883 |
| institution | PANGAEA |
| language | en |
| publishDate | 2015 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 Wei, Lei Wang, Qing Ning, Xuanxuan Mu, Changkao Wang, Chunlin Cao, Ruiwen Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; South Pacific; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. |
| title | Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; South Pacific; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment |
| url | https://doi.org/10.1594/PANGAEA.846883 |