(Table 2) Rate of reduction in cell size (apical length) and growth rate for seven Fragilariopsis kerguelensis strains sampled during POLARSTERN cruise ANT-XXI/3
Fuente:
PANGAEA
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Dataset Open Access |
| Sprache: | en |
| Veröffentlicht: |
PANGAEA
2013
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1867168167919353856 |
|---|---|
| author | Fuchs, Nike Scalco, Eleonora Kooistra, W H C F Assmy, Philipp Montresor, Marina |
| author_facet | Fuchs, Nike Scalco, Eleonora Kooistra, W H C F Assmy, Philipp Montresor, Marina |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The planktonic diatom Fragilariopsis kerguelensis plays an important role in the biogeochemical cycles of the Southern Ocean, where remains of its frustules form the largest deposit of biogenic silica anywhere in the world. We assessed the genetic identity of 26 strains, from cells collected at various sites in the Southern Ocean, using three molecular markers, LSU and ITS rDNA and rbcL. The LSU sequences were identical among the tested strains, ITS sequences were highly similar, and only one base pair difference was detected among the rbcL sequences. These results, together with a large number of successful mating experiments demonstrated that the strains belong to a single biological species. We investigated the mating system and life cycle traits of F. kerguelensis. Cell size diminished gradually in clonal strains. Gamete formation only occurred when strains of opposite mating type - within a cell size range of 7-36 µm - were mixed together. Two binucleate gametes were formed in each gametangium and gamete conjugation produced a zygote that had four nuclei and was surrounded by thin siliceous scales. Two out of the four nuclei subsequently degenerated and the zygote expanded to form an auxospore surrounded by a transverse and a longitudinal perizonium. Staining with the fluorochrome PDMPO provided for the first time a clear demonstration that the longitudinal perizonium is formed after auxospore expansion is complete. Initial cells produced within the mature auxospores were 78-101 µm in length. Various authors have shown that the average valve size of F. kerguelensis varies in sediment samples collected in regions and seasons with different primary production regimes and this parameter has thus been proposed as a biological proxy for palaeo-productivity. A better understanding of the life cycle of F. kerguelensis should help the design of future investigations aimed at testing the link between cell size distribution in the natural environment and the role that environmental factors might have in the regulation of population cell size. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_847739 |
| institution | PANGAEA |
| language | en |
| publishDate | 2013 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | (Table 2) Rate of reduction in cell size (apical length) and growth rate for seven Fragilariopsis kerguelensis strains sampled during POLARSTERN cruise ANT-XXI/3 Fuchs, Nike Scalco, Eleonora Kooistra, W H C F Assmy, Philipp Montresor, Marina ANT-XXI/3; AWI; Cell division rate; Cell size; Cell size decrease; Cell size decrease per division; Date/Time of event; Event label; Hand net; HN; Latitude of event; Longitude of event; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS65/410-2; PS65/420-4; PS65/424-4; PS65 EIFEX; South Atlantic Ocean; Species; SPP1158; Strain The planktonic diatom Fragilariopsis kerguelensis plays an important role in the biogeochemical cycles of the Southern Ocean, where remains of its frustules form the largest deposit of biogenic silica anywhere in the world. We assessed the genetic identity of 26 strains, from cells collected at various sites in the Southern Ocean, using three molecular markers, LSU and ITS rDNA and rbcL. The LSU sequences were identical among the tested strains, ITS sequences were highly similar, and only one base pair difference was detected among the rbcL sequences. These results, together with a large number of successful mating experiments demonstrated that the strains belong to a single biological species. We investigated the mating system and life cycle traits of F. kerguelensis. Cell size diminished gradually in clonal strains. Gamete formation only occurred when strains of opposite mating type - within a cell size range of 7-36 µm - were mixed together. Two binucleate gametes were formed in each gametangium and gamete conjugation produced a zygote that had four nuclei and was surrounded by thin siliceous scales. Two out of the four nuclei subsequently degenerated and the zygote expanded to form an auxospore surrounded by a transverse and a longitudinal perizonium. Staining with the fluorochrome PDMPO provided for the first time a clear demonstration that the longitudinal perizonium is formed after auxospore expansion is complete. Initial cells produced within the mature auxospores were 78-101 µm in length. Various authors have shown that the average valve size of F. kerguelensis varies in sediment samples collected in regions and seasons with different primary production regimes and this parameter has thus been proposed as a biological proxy for palaeo-productivity. A better understanding of the life cycle of F. kerguelensis should help the design of future investigations aimed at testing the link between cell size distribution in the natural environment and the role that environmental factors might have in the regulation of population cell size. |
| title | (Table 2) Rate of reduction in cell size (apical length) and growth rate for seven Fragilariopsis kerguelensis strains sampled during POLARSTERN cruise ANT-XXI/3 |
| topic | ANT-XXI/3; AWI; Cell division rate; Cell size; Cell size decrease; Cell size decrease per division; Date/Time of event; Event label; Hand net; HN; Latitude of event; Longitude of event; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS65/410-2; PS65/420-4; PS65/424-4; PS65 EIFEX; South Atlantic Ocean; Species; SPP1158; Strain |
| url | https://doi.org/10.1594/PANGAEA.847739 |