Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua)
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2015
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| _version_ | 1867169106302599168 |
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| author | Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
| author_facet | Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was reduced by 30% below rates of control fish. This contrasted in vitro capacities of Na+/K+-ATPase, which remained unchanged under elevated CO2 at 10 °C, and earlier studies which had found a strong upregulation under severe hypercapnia. F1Fo-ATPase capacities increased in hypercapnic gills at both temperatures, whereas Na+/K+ATPase and H+-ATPase capacities only increased in response to elevated CO2 and warming indicating the absence of thermal compensation under CO2. We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. This effect is maintained at elevated temperature. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_848599 |
| institution | PANGAEA |
| language | en |
| publishDate | 2015 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) Kreiss, Cornelia M Michael, Katharina Bock, Christian Lucassen, Magnus Pörtner, Hans-Otto Adenosine triphosphatase activity; Alkalinity, total; Animalia; Aragonite saturation state; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Calcite saturation state; Calculated; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Coast and continental shelf; Concentration; Containers and aquaria (20-1000 L or < 1 m**2); CTD, Sea-Bird SBE 911plus; Description; Enzyme; Figure; Fraction; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gadus morhua; Laboratory experiment; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Sodium/Potassium adenosine triphosphatase activity; Species; Standard deviation; Temperate; Temperature; Temperature, water; Temperature, water, standard deviation; Tissues; Treatment: temperature Effects of severe hypercapnia have been extensively studied in marine fishes, while knowledge on the impacts of moderately elevated CO2 levels and their combination with warming is scarce. Here we investigate ion regulation mechanisms and energy budget in gills from Atlantic cod acclimated long-term to elevated PCO2 levels (2500 µatm) and temperature (18 °C). Isolated perfused gill preparations established to determine gill thermal plasticity during acute exposures (10-22 °C) and in vivo costs of Na+/K+-ATPase activity, protein and RNA synthesis. Maximum enzyme capacities of F1Fo-ATPase, H+-ATPase and Na+/K+-ATPase were measured in vitro in crude gill homogenates. After whole animal acclimation to elevated PCO2 and/or warming, branchial oxygen consumption responded more strongly to acute temperature change. The fractions of gill respiration allocated to protein and RNA synthesis remained unchanged. In gills of fish CO2-exposed at both temperatures, energy turnover associated with Na+/K+-ATPase activity was reduced by 30% below rates of control fish. This contrasted in vitro capacities of Na+/K+-ATPase, which remained unchanged under elevated CO2 at 10 °C, and earlier studies which had found a strong upregulation under severe hypercapnia. F1Fo-ATPase capacities increased in hypercapnic gills at both temperatures, whereas Na+/K+ATPase and H+-ATPase capacities only increased in response to elevated CO2 and warming indicating the absence of thermal compensation under CO2. We conclude that in vivo ion regulatory energy demand is lowered under moderately elevated CO2 levels despite the stronger thermal response of total gill respiration and the upregulation of F1Fo-ATPase. This effect is maintained at elevated temperature. |
| title | Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua) |
| topic | Adenosine triphosphatase activity; Alkalinity, total; Animalia; Aragonite saturation state; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Calcite saturation state; Calculated; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Coast and continental shelf; Concentration; Containers and aquaria (20-1000 L or < 1 m**2); CTD, Sea-Bird SBE 911plus; Description; Enzyme; Figure; Fraction; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gadus morhua; Laboratory experiment; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Sodium/Potassium adenosine triphosphatase activity; Species; Standard deviation; Temperate; Temperature; Temperature, water; Temperature, water, standard deviation; Tissues; Treatment: temperature |
| url | https://doi.org/10.1594/PANGAEA.848599 |