Extracellular carbonic anhydrase concentration at the sea-surface microlayer and 1 m depth during METEOR cruise M117, Baltic Sea
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2016
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| _version_ | 1867168174144749568 |
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| author | Mustaffa, Nur Ili Hamizah |
| author_facet | Mustaffa, Nur Ili Hamizah |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We developed an effective fluorometric technique to quantify extracellular carbonic anhydrase (eCA) present in natural seawater samples. The technique includes the separation of eCA from cells to achieve low detection limits through high signal : noise ratios. eCA was efficiently extracted from cell membranes by treatment with 0.1 M phosphate buffer containing 2.5 M NaCl. The free eCA specifically forms a fluorescent complex with dansylamide, and the detection limit of the complex is below 0.1 nM. We applied the technique to samples from different culture solutions and natural seawater collected from the Baltic Sea. We observed eCA concentrations to be in the range of 0.10-0.67 nM in natural seawater. The data indicated that this technique is very sensitive, accurate, and feasible for routine and shipboard measurement of eCA from natural seawater. It is therefore an effective and rapid tool to investigate the carbon acquisition of phytoplankton both in mono culture as well natural communities. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_861762 |
| institution | PANGAEA |
| language | en |
| publishDate | 2016 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Extracellular carbonic anhydrase concentration at the sea-surface microlayer and 1 m depth during METEOR cruise M117, Baltic Sea Mustaffa, Nur Ili Hamizah Baltic Sea; Buoy; BUOY; Chlorophyll a; DATE/TIME; DEPTH, water; Enrichment factor; Event label; Extracellular carbonic anhydrase; Gulf of Finland; Latitude of event; Longitude of event; M117; M117_607-11; M117_608-9; M117_609-9; M117_614-24; M117_618-10; M117_619-8; M117_623-23; M117_629-9; Meteor (1986); Ratio; Station label We developed an effective fluorometric technique to quantify extracellular carbonic anhydrase (eCA) present in natural seawater samples. The technique includes the separation of eCA from cells to achieve low detection limits through high signal : noise ratios. eCA was efficiently extracted from cell membranes by treatment with 0.1 M phosphate buffer containing 2.5 M NaCl. The free eCA specifically forms a fluorescent complex with dansylamide, and the detection limit of the complex is below 0.1 nM. We applied the technique to samples from different culture solutions and natural seawater collected from the Baltic Sea. We observed eCA concentrations to be in the range of 0.10-0.67 nM in natural seawater. The data indicated that this technique is very sensitive, accurate, and feasible for routine and shipboard measurement of eCA from natural seawater. It is therefore an effective and rapid tool to investigate the carbon acquisition of phytoplankton both in mono culture as well natural communities. |
| title | Extracellular carbonic anhydrase concentration at the sea-surface microlayer and 1 m depth during METEOR cruise M117, Baltic Sea |
| topic | Baltic Sea; Buoy; BUOY; Chlorophyll a; DATE/TIME; DEPTH, water; Enrichment factor; Event label; Extracellular carbonic anhydrase; Gulf of Finland; Latitude of event; Longitude of event; M117; M117_607-11; M117_608-9; M117_609-9; M117_614-24; M117_618-10; M117_619-8; M117_623-23; M117_629-9; Meteor (1986); Ratio; Station label |
| url | https://doi.org/10.1594/PANGAEA.861762 |