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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2016
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| Online-Zugang: | https://doi.org/10.1594/PANGAEA.861965 |
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| _version_ | 1867170065876516864 |
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| author | Endo, H Sugie, Koji Yoshimura, T Suzuki, Koji |
| author_facet | Endo, H Sugie, Koji Yoshimura, T Suzuki, Koji |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Next-generation sequencing (NGS) technologies have enabled us to determine phytoplankton community compositions at high resolution. However, few studies have adopted this approach to assess the responses of natural phytoplankton communities to environmental change. Here, we report the impact of different CO2 levels on spring diatoms in the Oyashio region of the western North Pacific as estimated by NGS of the diatom-specific rbcL gene (DNA), which encodes the large subunit of RubisCO. We also examined the abundance and composition of rbcL transcripts (cDNA) in diatoms to assess their physiological responses to changing CO2 levels. A short-term (3-day) incubation experiment was carried out on-deck using surface Oyashio waters under different pCO2 levels (180, 350, 750, and 1000 µatm) in May 2011. During the incubation, the transcript abundance of the diatom-specific rbcL gene decreased with an increase in seawater pCO2 levels. These results suggest that CO2 fixation capacity of diatoms decreased rapidly under elevated CO2 levels. In the high CO2 treatments (750 and 1000 µatm), diversity of diatom-specific rbcL gene and its transcripts decreased relative to the control treatment (350µatm), as well as contributions of Chaetocerataceae, Thalassiosiraceae, and Fragilariaceae to the total population, but the contributions of Bacillariaceae increased. In the low CO2 treatment, contributions of Bacillariaceae also increased together with other eukaryotes. These suggest that changes in CO2 levels can alter the community composition of spring diatoms in the Oyashio region. Overall, the NGS technology provided us a deeper understanding of the response of diatoms to changes in CO2 levels in terms of their community composition, diversity, and photosynthetic physiology. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_861965 |
| institution | PANGAEA |
| language | en |
| publishDate | 2016 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Response of spring diatoms to CO2 availability in the Western North Pacific as determined by next-generation sequencing Endo, H Sugie, Koji Yoshimura, T Suzuki, Koji Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CHEMTAX (Lewitus et al., 2005); Chlorophyll a; Chlorophyll a, standard deviation; Community composition and diversity; Confidence interval; Containers and aquaria (20-1000 L or < 1 m**2); Contribution; Entire community; EXP; Experiment; Fluorescence-based quantitative real-time PCR (qPCR); Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Group; Growth/Morphology; Growth rate; Growth rate, standard deviation; High Performance Liquid Chromatography (HPLC); Incubation duration; Laboratory experiment; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Next-generation sequencing (NGS); Nitrate; Nitrate, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Open ocean; Operational taxonomic unit; Oyashio; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Percentage; pH, standard deviation; pH, total scale; Phosphate; Phosphate, standard deviation; Potentiometric titration; rbcL gene, copy number; rbcL gene, copy number, standard deviation; rbcL transcript, copy number; rbcL transcript, copy number, standard deviation; Salinity; Shannon Diversity Index; Silicate; Silicate, standard deviation; Simpson index of diversity; Taxon/taxa; Temperate; Temperature, water; Treatment; Type Next-generation sequencing (NGS) technologies have enabled us to determine phytoplankton community compositions at high resolution. However, few studies have adopted this approach to assess the responses of natural phytoplankton communities to environmental change. Here, we report the impact of different CO2 levels on spring diatoms in the Oyashio region of the western North Pacific as estimated by NGS of the diatom-specific rbcL gene (DNA), which encodes the large subunit of RubisCO. We also examined the abundance and composition of rbcL transcripts (cDNA) in diatoms to assess their physiological responses to changing CO2 levels. A short-term (3-day) incubation experiment was carried out on-deck using surface Oyashio waters under different pCO2 levels (180, 350, 750, and 1000 µatm) in May 2011. During the incubation, the transcript abundance of the diatom-specific rbcL gene decreased with an increase in seawater pCO2 levels. These results suggest that CO2 fixation capacity of diatoms decreased rapidly under elevated CO2 levels. In the high CO2 treatments (750 and 1000 µatm), diversity of diatom-specific rbcL gene and its transcripts decreased relative to the control treatment (350µatm), as well as contributions of Chaetocerataceae, Thalassiosiraceae, and Fragilariaceae to the total population, but the contributions of Bacillariaceae increased. In the low CO2 treatment, contributions of Bacillariaceae also increased together with other eukaryotes. These suggest that changes in CO2 levels can alter the community composition of spring diatoms in the Oyashio region. Overall, the NGS technology provided us a deeper understanding of the response of diatoms to changes in CO2 levels in terms of their community composition, diversity, and photosynthetic physiology. |
| title | Response of spring diatoms to CO2 availability in the Western North Pacific as determined by next-generation sequencing |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CHEMTAX (Lewitus et al., 2005); Chlorophyll a; Chlorophyll a, standard deviation; Community composition and diversity; Confidence interval; Containers and aquaria (20-1000 L or < 1 m**2); Contribution; Entire community; EXP; Experiment; Fluorescence-based quantitative real-time PCR (qPCR); Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Group; Growth/Morphology; Growth rate; Growth rate, standard deviation; High Performance Liquid Chromatography (HPLC); Incubation duration; Laboratory experiment; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Next-generation sequencing (NGS); Nitrate; Nitrate, standard deviation; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Open ocean; Operational taxonomic unit; Oyashio; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Percentage; pH, standard deviation; pH, total scale; Phosphate; Phosphate, standard deviation; Potentiometric titration; rbcL gene, copy number; rbcL gene, copy number, standard deviation; rbcL transcript, copy number; rbcL transcript, copy number, standard deviation; Salinity; Shannon Diversity Index; Silicate; Silicate, standard deviation; Simpson index of diversity; Taxon/taxa; Temperate; Temperature, water; Treatment; Type |
| url | https://doi.org/10.1594/PANGAEA.861965 |