Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa beta and toll-like receptor pathways
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2016
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| _version_ | 1867168174264287232 |
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| author | Liu, Saixi Shi, Wei Guo, Cheng Zhao, Xinguo Han, Yu Peng, Chao Chai, Xueliang Liu, Guangxu |
| author_facet | Liu, Saixi Shi, Wei Guo, Cheng Zhao, Xinguo Han, Yu Peng, Chao Chai, Xueliang Liu, Guangxu |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The impact of pCO2 driven ocean acidification on marine bivalve immunity remains poorly understood. To date, this impact has only been investigated in a few bivalve species and the underlying molecular mechanism remains unknown. In the present study, the effects of the realistic future ocean pCO2 levels (pH at 8.1, 7.8, and 7.4) on the total number of haemocyte cells (THC), phagocytosis status, blood cell types composition, and expression levels of twelve genes from the NF-kappa beta signaling and toll-like receptor pathways of a typical bottom burrowing bivalve, blood clam (Tegillarca granosa), were investigated. The results obtained showed that while both THC number and phagocytosis frequency were significantly reduced, the percentage of red and basophil granulocytes were significantly decreased and increased, respectively, upon exposure to elevated pCO2. In addition, exposure to pCO2 acidified seawater generally led to a significant down-regulation in the inducer and key response genes of NF-kappa beta signaling and toll-like receptor pathways. The results of the present study revealed that ocean acidification may hamper immune responses of the bivalve T. granosa which subsequently render individuals more susceptible to pathogens attacks such as those from virus and bacteria. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_861971 |
| institution | PANGAEA |
| language | en |
| publishDate | 2016 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa beta and toll-like receptor pathways Liu, Saixi Shi, Wei Guo, Cheng Zhao, Xinguo Han, Yu Peng, Chao Chai, Xueliang Liu, Guangxu Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Benthic animals; Benthos; Bicarbonate ion; Brackish waters; Calcite saturation state; Calcite saturation state, standard error; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Date; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Growth/Morphology; Height; Height, standard error; Hemocyte count; Hemocyte count, standard error; Immunology/Self-protection; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard error; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Percentage; Percentage, standard error; pH, NBS scale; pH, standard error; pH, total scale; Phagocytosis rate; Phagocytosis rate, standard error; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Salinity, standard error; Single species; Species; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Tissues; Treatment; Type; Uniform resource locator/link to reference The impact of pCO2 driven ocean acidification on marine bivalve immunity remains poorly understood. To date, this impact has only been investigated in a few bivalve species and the underlying molecular mechanism remains unknown. In the present study, the effects of the realistic future ocean pCO2 levels (pH at 8.1, 7.8, and 7.4) on the total number of haemocyte cells (THC), phagocytosis status, blood cell types composition, and expression levels of twelve genes from the NF-kappa beta signaling and toll-like receptor pathways of a typical bottom burrowing bivalve, blood clam (Tegillarca granosa), were investigated. The results obtained showed that while both THC number and phagocytosis frequency were significantly reduced, the percentage of red and basophil granulocytes were significantly decreased and increased, respectively, upon exposure to elevated pCO2. In addition, exposure to pCO2 acidified seawater generally led to a significant down-regulation in the inducer and key response genes of NF-kappa beta signaling and toll-like receptor pathways. The results of the present study revealed that ocean acidification may hamper immune responses of the bivalve T. granosa which subsequently render individuals more susceptible to pathogens attacks such as those from virus and bacteria. |
| title | Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa beta and toll-like receptor pathways |
| topic | Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Benthic animals; Benthos; Bicarbonate ion; Brackish waters; Calcite saturation state; Calcite saturation state, standard error; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Date; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Growth/Morphology; Height; Height, standard error; Hemocyte count; Hemocyte count, standard error; Immunology/Self-protection; Laboratory experiment; Mollusca; mRNA gene expression, relative; mRNA gene expression, relative, standard error; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Percentage; Percentage, standard error; pH, NBS scale; pH, standard error; pH, total scale; Phagocytosis rate; Phagocytosis rate, standard error; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Salinity, standard error; Single species; Species; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Tissues; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.861971 |