_version_ 1867170995465355264
author Shen, Sara G
Chen, Fangyi
Schoppik, David E
Checkley, D M Jr
author_facet Shen, Sara G
Chen, Fangyi
Schoppik, David E
Checkley, D M Jr
collection Datos científicos de ciencias marinas y ambientales
contents We investigated vestibular function and otolith size (OS) in larvae of white seabass Atractoscion nobilis exposed to high partial pressure of CO2 (pCO2). The context for our study is the increasing concentration of CO2 in seawater that is causing ocean acidification (OA). The utricular otoliths are aragonitic structures in the inner ear of fish that act to detect orientation and acceleration. Stimulation of the utricular otoliths during head movement results in a behavioral response called the vestibulo-ocular reflex (VOR). The VOR is a compensatory eye rotation that serves to maintain a stable image during movement. VOR is characterized by gain (ratio of eye amplitude to head amplitude) and phase shift (temporal synchrony). We hypothesized that elevated pCO2 would increase OS and affect the VOR. We found that the sagittae and lapilli of young larvae reared at 2500 µatm pCO2 (treatment) were 14 to 20% and 37 to 39% larger in area, respectively, than those of larvae reared at 400 µatm pCO2 (control). The mean gain of treatment larvae (0.39 +/- 0.05, n = 28) was not statistically different from that of control larvae (0.30 +/- 0.03, n = 20), although there was a tendency for treatment larvae to have a larger gain. Phase shift was unchanged. Our lack of detection of a significant effect of elevated pCO2 on the VOR may be a result of the low turbulence conditions of the experiments, large natural variation in otolith size, calibration of the VOR or mechanism of acid?base regulation of white seabass larvae.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_869806
institution PANGAEA
language en
publishDate 2016
publisher PANGAEA
record_format pangaea
spellingShingle Otolith size and the vestibulo-ocular reflex of larvae of white seabass Atractoscion nobilis at high pCO2
Shen, Sara G
Chen, Fangyi
Schoppik, David E
Checkley, D M Jr
Alkalinity, total; Animalia; Aragonite saturation state; Atractoscion nobilis; Behaviour; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Coast and continental shelf; Coulometric titration; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gain; Gain, standard error; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Otolith area; Otolith area, standard error; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, total scale; Phase shift; Phase shift, standard error; Potentiometric titration; Registration number of species; Replicates; Salinity; Single species; Species; Temperate; Temperature, water; Type; Uniform resource locator/link to reference
We investigated vestibular function and otolith size (OS) in larvae of white seabass Atractoscion nobilis exposed to high partial pressure of CO2 (pCO2). The context for our study is the increasing concentration of CO2 in seawater that is causing ocean acidification (OA). The utricular otoliths are aragonitic structures in the inner ear of fish that act to detect orientation and acceleration. Stimulation of the utricular otoliths during head movement results in a behavioral response called the vestibulo-ocular reflex (VOR). The VOR is a compensatory eye rotation that serves to maintain a stable image during movement. VOR is characterized by gain (ratio of eye amplitude to head amplitude) and phase shift (temporal synchrony). We hypothesized that elevated pCO2 would increase OS and affect the VOR. We found that the sagittae and lapilli of young larvae reared at 2500 µatm pCO2 (treatment) were 14 to 20% and 37 to 39% larger in area, respectively, than those of larvae reared at 400 µatm pCO2 (control). The mean gain of treatment larvae (0.39 +/- 0.05, n = 28) was not statistically different from that of control larvae (0.30 +/- 0.03, n = 20), although there was a tendency for treatment larvae to have a larger gain. Phase shift was unchanged. Our lack of detection of a significant effect of elevated pCO2 on the VOR may be a result of the low turbulence conditions of the experiments, large natural variation in otolith size, calibration of the VOR or mechanism of acid?base regulation of white seabass larvae.
title Otolith size and the vestibulo-ocular reflex of larvae of white seabass Atractoscion nobilis at high pCO2
topic Alkalinity, total; Animalia; Aragonite saturation state; Atractoscion nobilis; Behaviour; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; Coast and continental shelf; Coulometric titration; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gain; Gain, standard error; Growth/Morphology; Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Otolith area; Otolith area, standard error; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, total scale; Phase shift; Phase shift, standard error; Potentiometric titration; Registration number of species; Replicates; Salinity; Single species; Species; Temperate; Temperature, water; Type; Uniform resource locator/link to reference
url https://doi.org/10.1594/PANGAEA.869806