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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2016
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1594/PANGAEA.873048 |
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| _version_ | 1867170073504907264 |
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| author | Goncalves, Priscila Anderson, Kelli Thompson, Emma L Melwani, Aroon Parker, Laura M Raftos, David A |
| author_facet | Goncalves, Priscila Anderson, Kelli Thompson, Emma L Melwani, Aroon Parker, Laura M Raftos, David A |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Marine organisms need to adapt in order to cope with the adverse effects of ocean acidification and warming. Transgenerational exposure to CO2 stress has been shown to enhance resilience to ocean acidification in offspring from a number of species. However, the molecular basis underlying such adaptive responses is currently unknown. Here, we compared the transcriptional profiles of two genetically distinct oyster breeding lines following transgenerational exposure to elevated CO2 in order to explore the molecular basis of acclimation or adaptation to ocean acidification in these organisms. The expression of key target genes associated with antioxidant defence, metabolism and the cytoskeleton was assessed in oysters exposed to elevated CO2 over three consecutive generations. This set of target genes was chosen specifically to test whether altered responsiveness of intracellular stress responses contributes to the differential acclimation of oyster populations to climate stressors. Transgenerational exposure to elevated CO2 resulted in changes to both basal and inducible expression of those key target genes (e.g. ecSOD, catalase and peroxiredoxin 6), particularly in oysters derived from the disease-resistant, fast-growing B2 line. Exposure to CO2 stress over consecutive generations produced opposite and less evident effects on transcription in a second population that was derived from wild type (non-selected) oysters. The analysis of key target genes revealed that the acute responses of oysters to CO2 stress appear to be affected by population-specific genetic and/or phenotypic traits, and by the CO2 conditions to which their parents had been exposed. This supports the contention that the capacity for heritable change in response to ocean acidification varies between oyster breeding lines and is mediated by parental conditioning. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_873048 |
| institution | PANGAEA |
| language | en |
| publishDate | 2016 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Rapid transcriptional acclimation following transgenerational exposure of oysters to ocean acidification Goncalves, Priscila Anderson, Kelli Thompson, Emma L Melwani, Aroon Parker, Laura M Raftos, David A Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Description; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Identification; Laboratory experiment; Laboratory strains; Mollusca; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Real-time polymerase chain reaction counts; Real-time polymerase chain reaction counts, standard deviation; Registration number of species; Saccostrea glomerata; Salinity; Salinity, standard deviation; Sample ID; Single species; South Pacific; Species; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference Marine organisms need to adapt in order to cope with the adverse effects of ocean acidification and warming. Transgenerational exposure to CO2 stress has been shown to enhance resilience to ocean acidification in offspring from a number of species. However, the molecular basis underlying such adaptive responses is currently unknown. Here, we compared the transcriptional profiles of two genetically distinct oyster breeding lines following transgenerational exposure to elevated CO2 in order to explore the molecular basis of acclimation or adaptation to ocean acidification in these organisms. The expression of key target genes associated with antioxidant defence, metabolism and the cytoskeleton was assessed in oysters exposed to elevated CO2 over three consecutive generations. This set of target genes was chosen specifically to test whether altered responsiveness of intracellular stress responses contributes to the differential acclimation of oyster populations to climate stressors. Transgenerational exposure to elevated CO2 resulted in changes to both basal and inducible expression of those key target genes (e.g. ecSOD, catalase and peroxiredoxin 6), particularly in oysters derived from the disease-resistant, fast-growing B2 line. Exposure to CO2 stress over consecutive generations produced opposite and less evident effects on transcription in a second population that was derived from wild type (non-selected) oysters. The analysis of key target genes revealed that the acute responses of oysters to CO2 stress appear to be affected by population-specific genetic and/or phenotypic traits, and by the CO2 conditions to which their parents had been exposed. This supports the contention that the capacity for heritable change in response to ocean acidification varies between oyster breeding lines and is mediated by parental conditioning. |
| title | Rapid transcriptional acclimation following transgenerational exposure of oysters to ocean acidification |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Description; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gene name; Identification; Laboratory experiment; Laboratory strains; Mollusca; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Real-time polymerase chain reaction counts; Real-time polymerase chain reaction counts, standard deviation; Registration number of species; Saccostrea glomerata; Salinity; Salinity, standard deviation; Sample ID; Single species; South Pacific; Species; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.873048 |