Sediment bacterial community in sandy sediment at CO2 natural vents in Basiluzzo (Panarea, Italy): sediment raw ARISA (Automated Ribosomal Intergenic Spacer Analysis) data
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PANGAEA
2017
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| _version_ | 1867168460465766400 |
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| author | Molari, Massimiliano |
| author_facet | Molari, Massimiliano |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Sediment bacterial community at natural CO2 vents as described applying molecular fingerprint technique Automated Ribosomal Intergenic Spacer Analysis (ARISA) on sandy sediment samples collected in 2011, 2012 and 2013 collected at Basiluzzo Islet (Panarea Island). Automated ribosomal intergenic spacer analysis (ARISA) is a quantitative fingerprinting approach for estimating the abundance of Operation Taxonomic Units (OUT) in natural microbial communities. A detailed description of this molecular method is provided in Ramette (2009)*. Briefly PCR-amplified fragments, obtained using primes that yield peaks in the range 100 to 1,000 bp, were separated via capillary electrophoresis and then raw profiles were checked for stable baselines and voltage, and peak sizes and absolute areas were determined by using GeneMapper software v 3.7 (Applied Biosystems) with minimum peak heights of 50 fluorescence units for all dyes. Here the GeneMapper output table is provided. In Molari et al. (XXXX) samples-by-binned-OUT tables was obtained by calculating relative fluorescence intensity (RFI), considering fragments above a threshold of 50 fluorescence units and between 100–1000 bp length, and applying a binning strategy with a bin size of 2 bp. *Ramette, Alban (2009). Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities. Applied and Environmental Microbiology, 75(8): 2495−2505. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_873500 |
| institution | PANGAEA |
| language | en |
| publishDate | 2017 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Sediment bacterial community in sandy sediment at CO2 natural vents in Basiluzzo (Panarea, Italy): sediment raw ARISA (Automated Ribosomal Intergenic Spacer Analysis) data Molari, Massimiliano Base pair size; Date/Time of event; ECO2; ECO2-2; ECO2-2-FT-1; ECO2-2-FT-11; ECO2-2-FT-12; ECO2-2-FT-2; ECO2-2-FT-27; ECO2-2-FT-28; ECO2-2-FT-29; ECO2-2-FT-3; ECO2-2-FT-30; ECO2-2-FT-31; ECO2-2-FT-32; ECO2-2-FT-33; ECO2-2-FT-34; ECO2-2-FT-35; ECO2-2-FT-36; ECO2-2-FT-37; ECO2-2-FT-38; ECO2-2-FT-4; ECO2-2-FT-5; ECO2-2-FT-53; ECO2-2-FT-54; ECO2-2-FT-55; ECO2-2-FT-56; ECO2-2-FT-57; ECO2-2-FT-58; ECO2-2-FT-59; ECO2-2-FT-6; ECO2-2-FT-60; ECO2-2-FT-61; ECO2-2-FT-62; ECO2-2-FT-63; ECO2-2-FT-64; ECO2-2-FT-7; ECO2-2-FT-8; ECO2-2-PUC-1a; ECO2-2-PUC-1b; ECO2-2-PUC-1c; ECO2-2-PUC-2a; ECO2-2-PUC-2b; ECO2-2-PUC-2c; ECO2-3; ECO2-3-FT-1; ECO2-3-FT-10; ECO2-3-FT-11; ECO2-3-FT-12; ECO2-3-FT-13; ECO2-3-FT-14; ECO2-3-FT-15; ECO2-3-FT-16; ECO2-3-FT-18; ECO2-3-FT-19; ECO2-3-FT-2; ECO2-3-FT-20; ECO2-3-FT-3; ECO2-3-FT-36; ECO2-3-FT-37; ECO2-3-FT-38; ECO2-3-FT-39; ECO2-3-FT-4; ECO2-3-FT-40; ECO2-3-FT-41; ECO2-3-FT-42; ECO2-3-FT-43; ECO2-3-FT-44; ECO2-3-FT-45; ECO2-3-FT-46; ECO2-3-FT-47; ECO2-3-FT-48; ECO2-3-FT-49; ECO2-3-FT-5; ECO2-3-FT-50; ECO2-3-FT-51; ECO2-3-FT-52; ECO2-3-FT-53; ECO2-3-FT-54; ECO2-3-FT-55; ECO2-3-FT-6; ECO2-3-FT-7; ECO2-3-FT-71; ECO2-3-FT-72; ECO2-3-FT-73; ECO2-3-FT-74; ECO2-3-FT-75; ECO2-3-FT-76; ECO2-3-FT-77; ECO2-3-FT-78; ECO2-3-FT-79; ECO2-3-FT-8; ECO2-3-FT-80; ECO2-3-FT-81; ECO2-3-FT-82; ECO2-3-FT-83; ECO2-3-FT-84; ECO2-3-FT-85; ECO2-3-FT-86; ECO2-3-FT-87; ECO2-3-FT-88; ECO2-3-FT-89; ECO2-3-FT-9; ECO2-3-FT-90; ECO2-3-PUC-10c; ECO2-3-PUC-11b; ECO2-3-PUC-12d; ECO2-3-PUC-13d; ECO2-3-PUC-13f; ECO2-3-PUC-16c; ECO2-3-PUC-17a; ECO2-3-PUC-1a; ECO2-3-PUC-2a; ECO2-3-PUC-2b; ECO2-3-PUC-2d; ECO2-3-PUC-3a; ECO2-3-PUC-3b; ECO2-3-PUC-3c; ECO2-3-PUC-4a; ECO2-3-PUC-4b; ECO2-3-PUC-4c; ECO2-3-PUC-5a; ECO2-3-PUC-5b; ECO2-3-PUC-6a; ECO2-3-PUC-6c; ECO2-3-PUC-6f; ECO2-3-PUC-7a; ECO2-3-PUC-8c; ECO2-3-PUC-8e; ECO2-3-PUC-9a; ECO2-3-PUC-9b; ECO2-8; ECO2-8-FT-1; ECO2-8-FT-10; ECO2-8-FT-11; ECO2-8-FT-12; ECO2-8-FT-13; ECO2-8-FT-14; ECO2-8-FT-15; ECO2-8-FT-16; ECO2-8-FT-17; ECO2-8-FT-18; ECO2-8-FT-19; ECO2-8-FT-2; ECO2-8-FT-20; ECO2-8-FT-21; ECO2-8-FT-22; ECO2-8-FT-23; ECO2-8-FT-24; ECO2-8-FT-25; ECO2-8-FT-26; ECO2-8-FT-27; ECO2-8-FT-28; ECO2-8-FT-29; ECO2-8-FT-3; ECO2-8-FT-30; ECO2-8-FT-31; ECO2-8-FT-32; ECO2-8-FT-33; ECO2-8-FT-34; ECO2-8-FT-35; ECO2-8-FT-36; ECO2-8-FT-37; ECO2-8-FT-38; ECO2-8-FT-39; ECO2-8-FT-4; ECO2-8-FT-40; ECO2-8-FT-41; ECO2-8-FT-42; ECO2-8-FT-43; ECO2-8-FT-44; ECO2-8-FT-45; ECO2-8-FT-46; ECO2-8-FT-47; ECO2-8-FT-48; ECO2-8-FT-49; ECO2-8-FT-5; ECO2-8-FT-50; ECO2-8-FT-51; ECO2-8-FT-52; ECO2-8-FT-53; ECO2-8-FT-54; ECO2-8-FT-55; ECO2-8-FT-56; ECO2-8-FT-57; ECO2-8-FT-58; ECO2-8-FT-59; ECO2-8-FT-6; ECO2-8-FT-60; ECO2-8-FT-7; ECO2-8-FT-8; ECO2-8-FT-9; ECO2-8-PUC-10a; ECO2-8-PUC-10b; ECO2-8-PUC-10c; ECO2-8-PUC-11a; ECO2-8-PUC-11b; ECO2-8-PUC-12a; ECO2-8-PUC-12b; ECO2-8-PUC-12c; ECO2-8-PUC-16a; ECO2-8-PUC-16b; ECO2-8-PUC-2a; ECO2-8-PUC-6a; ECO2-8-PUC-6b; ECO2-8-PUC-7a; ECO2-8-PUC-7b; ECO2-8-PUC-7c; ECO2-8-PUC-8a; ECO2-8-PUC-8b; ECO2-8-PUC-9a; ECO2-8-PUC-9b; ECO2-8-PUC-9c; Elevation of event; Event label; Hand push corer; HSR; Latitude of event; Longitude of event; Panarea; Peak area; PUC; Push corer; Sample code/label; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; Zodiac Sediment bacterial community at natural CO2 vents as described applying molecular fingerprint technique Automated Ribosomal Intergenic Spacer Analysis (ARISA) on sandy sediment samples collected in 2011, 2012 and 2013 collected at Basiluzzo Islet (Panarea Island). Automated ribosomal intergenic spacer analysis (ARISA) is a quantitative fingerprinting approach for estimating the abundance of Operation Taxonomic Units (OUT) in natural microbial communities. A detailed description of this molecular method is provided in Ramette (2009)*. Briefly PCR-amplified fragments, obtained using primes that yield peaks in the range 100 to 1,000 bp, were separated via capillary electrophoresis and then raw profiles were checked for stable baselines and voltage, and peak sizes and absolute areas were determined by using GeneMapper software v 3.7 (Applied Biosystems) with minimum peak heights of 50 fluorescence units for all dyes. Here the GeneMapper output table is provided. In Molari et al. (XXXX) samples-by-binned-OUT tables was obtained by calculating relative fluorescence intensity (RFI), considering fragments above a threshold of 50 fluorescence units and between 100–1000 bp length, and applying a binning strategy with a bin size of 2 bp. *Ramette, Alban (2009). Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities. Applied and Environmental Microbiology, 75(8): 2495−2505. |
| title | Sediment bacterial community in sandy sediment at CO2 natural vents in Basiluzzo (Panarea, Italy): sediment raw ARISA (Automated Ribosomal Intergenic Spacer Analysis) data |
| topic | Base pair size; Date/Time of event; ECO2; ECO2-2; ECO2-2-FT-1; ECO2-2-FT-11; ECO2-2-FT-12; ECO2-2-FT-2; ECO2-2-FT-27; ECO2-2-FT-28; ECO2-2-FT-29; ECO2-2-FT-3; ECO2-2-FT-30; ECO2-2-FT-31; ECO2-2-FT-32; ECO2-2-FT-33; ECO2-2-FT-34; ECO2-2-FT-35; ECO2-2-FT-36; ECO2-2-FT-37; ECO2-2-FT-38; ECO2-2-FT-4; ECO2-2-FT-5; ECO2-2-FT-53; ECO2-2-FT-54; ECO2-2-FT-55; ECO2-2-FT-56; ECO2-2-FT-57; ECO2-2-FT-58; ECO2-2-FT-59; ECO2-2-FT-6; ECO2-2-FT-60; ECO2-2-FT-61; ECO2-2-FT-62; ECO2-2-FT-63; ECO2-2-FT-64; ECO2-2-FT-7; ECO2-2-FT-8; ECO2-2-PUC-1a; ECO2-2-PUC-1b; ECO2-2-PUC-1c; ECO2-2-PUC-2a; ECO2-2-PUC-2b; ECO2-2-PUC-2c; ECO2-3; ECO2-3-FT-1; ECO2-3-FT-10; ECO2-3-FT-11; ECO2-3-FT-12; ECO2-3-FT-13; ECO2-3-FT-14; ECO2-3-FT-15; ECO2-3-FT-16; ECO2-3-FT-18; ECO2-3-FT-19; ECO2-3-FT-2; ECO2-3-FT-20; ECO2-3-FT-3; ECO2-3-FT-36; ECO2-3-FT-37; ECO2-3-FT-38; ECO2-3-FT-39; ECO2-3-FT-4; ECO2-3-FT-40; ECO2-3-FT-41; ECO2-3-FT-42; ECO2-3-FT-43; ECO2-3-FT-44; ECO2-3-FT-45; ECO2-3-FT-46; ECO2-3-FT-47; ECO2-3-FT-48; ECO2-3-FT-49; ECO2-3-FT-5; ECO2-3-FT-50; ECO2-3-FT-51; ECO2-3-FT-52; ECO2-3-FT-53; ECO2-3-FT-54; ECO2-3-FT-55; ECO2-3-FT-6; ECO2-3-FT-7; ECO2-3-FT-71; ECO2-3-FT-72; ECO2-3-FT-73; ECO2-3-FT-74; ECO2-3-FT-75; ECO2-3-FT-76; ECO2-3-FT-77; ECO2-3-FT-78; ECO2-3-FT-79; ECO2-3-FT-8; ECO2-3-FT-80; ECO2-3-FT-81; ECO2-3-FT-82; ECO2-3-FT-83; ECO2-3-FT-84; ECO2-3-FT-85; ECO2-3-FT-86; ECO2-3-FT-87; ECO2-3-FT-88; ECO2-3-FT-89; ECO2-3-FT-9; ECO2-3-FT-90; ECO2-3-PUC-10c; ECO2-3-PUC-11b; ECO2-3-PUC-12d; ECO2-3-PUC-13d; ECO2-3-PUC-13f; ECO2-3-PUC-16c; ECO2-3-PUC-17a; ECO2-3-PUC-1a; ECO2-3-PUC-2a; ECO2-3-PUC-2b; ECO2-3-PUC-2d; ECO2-3-PUC-3a; ECO2-3-PUC-3b; ECO2-3-PUC-3c; ECO2-3-PUC-4a; ECO2-3-PUC-4b; ECO2-3-PUC-4c; ECO2-3-PUC-5a; ECO2-3-PUC-5b; ECO2-3-PUC-6a; ECO2-3-PUC-6c; ECO2-3-PUC-6f; ECO2-3-PUC-7a; ECO2-3-PUC-8c; ECO2-3-PUC-8e; ECO2-3-PUC-9a; ECO2-3-PUC-9b; ECO2-8; ECO2-8-FT-1; ECO2-8-FT-10; ECO2-8-FT-11; ECO2-8-FT-12; ECO2-8-FT-13; ECO2-8-FT-14; ECO2-8-FT-15; ECO2-8-FT-16; ECO2-8-FT-17; ECO2-8-FT-18; ECO2-8-FT-19; ECO2-8-FT-2; ECO2-8-FT-20; ECO2-8-FT-21; ECO2-8-FT-22; ECO2-8-FT-23; ECO2-8-FT-24; ECO2-8-FT-25; ECO2-8-FT-26; ECO2-8-FT-27; ECO2-8-FT-28; ECO2-8-FT-29; ECO2-8-FT-3; ECO2-8-FT-30; ECO2-8-FT-31; ECO2-8-FT-32; ECO2-8-FT-33; ECO2-8-FT-34; ECO2-8-FT-35; ECO2-8-FT-36; ECO2-8-FT-37; ECO2-8-FT-38; ECO2-8-FT-39; ECO2-8-FT-4; ECO2-8-FT-40; ECO2-8-FT-41; ECO2-8-FT-42; ECO2-8-FT-43; ECO2-8-FT-44; ECO2-8-FT-45; ECO2-8-FT-46; ECO2-8-FT-47; ECO2-8-FT-48; ECO2-8-FT-49; ECO2-8-FT-5; ECO2-8-FT-50; ECO2-8-FT-51; ECO2-8-FT-52; ECO2-8-FT-53; ECO2-8-FT-54; ECO2-8-FT-55; ECO2-8-FT-56; ECO2-8-FT-57; ECO2-8-FT-58; ECO2-8-FT-59; ECO2-8-FT-6; ECO2-8-FT-60; ECO2-8-FT-7; ECO2-8-FT-8; ECO2-8-FT-9; ECO2-8-PUC-10a; ECO2-8-PUC-10b; ECO2-8-PUC-10c; ECO2-8-PUC-11a; ECO2-8-PUC-11b; ECO2-8-PUC-12a; ECO2-8-PUC-12b; ECO2-8-PUC-12c; ECO2-8-PUC-16a; ECO2-8-PUC-16b; ECO2-8-PUC-2a; ECO2-8-PUC-6a; ECO2-8-PUC-6b; ECO2-8-PUC-7a; ECO2-8-PUC-7b; ECO2-8-PUC-7c; ECO2-8-PUC-8a; ECO2-8-PUC-8b; ECO2-8-PUC-9a; ECO2-8-PUC-9b; ECO2-8-PUC-9c; Elevation of event; Event label; Hand push corer; HSR; Latitude of event; Longitude of event; Panarea; Peak area; PUC; Push corer; Sample code/label; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; Zodiac |
| url | https://doi.org/10.1594/PANGAEA.873500 |