Sequences of the 16S and 18S gene and pH determination of the Jena Experiment main plots measured in 2010
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2017
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| _version_ | 1867168570089144320 |
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| author | Dassen, Sigrid Cortois, Roeland Martens, H de Hollander, M Kowalchuk, George A van der Putten, Wim H De Deyn, Gerlinde B |
| author_facet | Dassen, Sigrid Cortois, Roeland Martens, H de Hollander, M Kowalchuk, George A van der Putten, Wim H De Deyn, Gerlinde B |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | In september 2010 bulk soil samples were taken from 82 plots in the Jena Experiment main experiment. Per plot 5 cores of 15 cm depth were taken, pooled and sieved. From the extracted DNA the 16S and 18S rRNA gene was amplified with primer sets 515f/806r and FR 1/FF390. The samples were subjected to Roche 454 automated sequencer and GS FLX system using titanium chemistry (Macrogen Seoul, Korea). The 16S dataset includes bacterial and archaeal sequences, the 18S dataset includes mainly fungal and protist sequences. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_874990 |
| institution | PANGAEA |
| language | en |
| publishDate | 2017 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Sequences of the 16S and 18S gene and pH determination of the Jena Experiment main plots measured in 2010 Dassen, Sigrid Cortois, Roeland Martens, H de Hollander, M Kowalchuk, George A van der Putten, Wim H De Deyn, Gerlinde B JenExp; The Jena Experiment In september 2010 bulk soil samples were taken from 82 plots in the Jena Experiment main experiment. Per plot 5 cores of 15 cm depth were taken, pooled and sieved. From the extracted DNA the 16S and 18S rRNA gene was amplified with primer sets 515f/806r and FR 1/FF390. The samples were subjected to Roche 454 automated sequencer and GS FLX system using titanium chemistry (Macrogen Seoul, Korea). The 16S dataset includes bacterial and archaeal sequences, the 18S dataset includes mainly fungal and protist sequences. |
| title | Sequences of the 16S and 18S gene and pH determination of the Jena Experiment main plots measured in 2010 |
| topic | JenExp; The Jena Experiment |
| url | https://doi.org/10.1594/PANGAEA.874990 |