Seawater carbonate chemistry and cadmium uptake of the smooth scallop Flexopecten glaber
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2018
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| author | Nardi, Alessandro Benedetti, Maura Fattorini, Daniele Regoli, Francesco |
| author_facet | Nardi, Alessandro Benedetti, Maura Fattorini, Daniele Regoli, Francesco |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification (OA) may affect sensitivity of marine organisms to metal pollution modulating chemical bioavailability, bioaccumulation and biological responsiveness of several cellular pathways. In this study, the smooth scallop Flexopecten glaber was exposed to various combinations of reduced pH (pH/pCO2 7.4/3000 µatm) and Cd (20 µg/L). The analyses on cadmium uptake were integrated with those of a wide battery of biomarkers including metallothioneins, single antioxidant defenses and total oxyradical scavenging capacity in digestive gland and gills, lysosomal membrane stability and onset of genotoxic damage in haemocytes. Reduced pH slightly increased concentration of Cd in scallop tissues, but no effects were measured in terms of metallothioneins. Induction of some antioxidants by Cd and/or low pH in the digestive gland was not reflected in variations of the total oxyradical scavenging capacity, while the investigated stressors caused a certain inhibition of antioxidants and reduction of the scavenging capacity toward peroxyl radical in the gills. Lysosomal membrane stability and onset of genotoxic damages showed high sensitivity with possible synergistic effects of the investigated factors. The overall results suggest that indirect effects of ocean acidification on metal accumulation and toxicity are tissue-specific and modulate oxidative balance through different mechanisms. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_887768 |
| institution | PANGAEA |
| language | en |
| publishDate | 2018 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and cadmium uptake of the smooth scallop Flexopecten glaber Nardi, Alessandro Benedetti, Maura Fattorini, Daniele Regoli, Francesco Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Cadmium; Cadmium, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Catalase activity, per protein mass; Catalase activity, standard deviation; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Experiment duration; Flexopecten glaber; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione, total, per unit wet mass; Glutathione, total, per unit wet mass, standard deviation; Glutathione reductase, per protein mass; Glutathione reductase, unit per protein mass, standard deviation; Glutathione S-transferase, activity per protein mass; Glutathione S-transferase, activity per protein mass, standard deviation; Hemocytes DNA strand breaks; Hemocytes DNA strand breaks, standard deviation; Hemocytes lysosomal membranes stability; Hemocytes lysosomal membranes stability, standard deviation; Hemocytes micronuclei frequency; Hemocytes micronuclei frequency, standard deviation; Identification; Immunology/Self-protection; Inorganic toxins; Laboratory experiment; Mediterranean Sea; Metallothionein, per protein mass; Metallothionein per protein, standard deviation; Mollusca; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Registration number of species; Salinity, standard deviation; Selenium dependent glutathione peroxidases activity, per protein mass; Selenium dependent glutathione peroxidases activity, unit per protein mass, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Total glutathione peroxidases activity, per protein mass; Total glutathione peroxidases activity, unit per protein mass, standard deviation; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard deviation; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard deviation; Treatment; Type; Uniform resource locator/link to reference Ocean acidification (OA) may affect sensitivity of marine organisms to metal pollution modulating chemical bioavailability, bioaccumulation and biological responsiveness of several cellular pathways. In this study, the smooth scallop Flexopecten glaber was exposed to various combinations of reduced pH (pH/pCO2 7.4/3000 µatm) and Cd (20 µg/L). The analyses on cadmium uptake were integrated with those of a wide battery of biomarkers including metallothioneins, single antioxidant defenses and total oxyradical scavenging capacity in digestive gland and gills, lysosomal membrane stability and onset of genotoxic damage in haemocytes. Reduced pH slightly increased concentration of Cd in scallop tissues, but no effects were measured in terms of metallothioneins. Induction of some antioxidants by Cd and/or low pH in the digestive gland was not reflected in variations of the total oxyradical scavenging capacity, while the investigated stressors caused a certain inhibition of antioxidants and reduction of the scavenging capacity toward peroxyl radical in the gills. Lysosomal membrane stability and onset of genotoxic damages showed high sensitivity with possible synergistic effects of the investigated factors. The overall results suggest that indirect effects of ocean acidification on metal accumulation and toxicity are tissue-specific and modulate oxidative balance through different mechanisms. |
| title | Seawater carbonate chemistry and cadmium uptake of the smooth scallop Flexopecten glaber |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Cadmium; Cadmium, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Catalase activity, per protein mass; Catalase activity, standard deviation; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Experiment duration; Flexopecten glaber; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione, total, per unit wet mass; Glutathione, total, per unit wet mass, standard deviation; Glutathione reductase, per protein mass; Glutathione reductase, unit per protein mass, standard deviation; Glutathione S-transferase, activity per protein mass; Glutathione S-transferase, activity per protein mass, standard deviation; Hemocytes DNA strand breaks; Hemocytes DNA strand breaks, standard deviation; Hemocytes lysosomal membranes stability; Hemocytes lysosomal membranes stability, standard deviation; Hemocytes micronuclei frequency; Hemocytes micronuclei frequency, standard deviation; Identification; Immunology/Self-protection; Inorganic toxins; Laboratory experiment; Mediterranean Sea; Metallothionein, per protein mass; Metallothionein per protein, standard deviation; Mollusca; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Registration number of species; Salinity, standard deviation; Selenium dependent glutathione peroxidases activity, per protein mass; Selenium dependent glutathione peroxidases activity, unit per protein mass, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Total glutathione peroxidases activity, per protein mass; Total glutathione peroxidases activity, unit per protein mass, standard deviation; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard deviation; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass; Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard deviation; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.887768 |