Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa
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| Main Authors: | , , , , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2017
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| _version_ | 1867171855290335232 |
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| author | Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu |
| author_facet | Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_888184 |
| institution | PANGAEA |
| language | en |
| publishDate | 2017 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu Alkalinity, total; Alkalinity, total, standard error; Amplitude; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Average path velocity; Average path velocity, standard error; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Curvilinear velocity; Curvilinear velocity, standard error; EXP; Experiment; Fertilization success rate; Fluorescence intensity; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; Potentiometric; Potentiometric titration; Probability; Registration number of species; Replicate; Reproduction; Salinity; Salinity, standard error; Single species; Species; Straight line velocity; Straight line velocity, standard error; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference; Yueqing_Bay Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations. |
| title | Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
| topic | Alkalinity, total; Alkalinity, total, standard error; Amplitude; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Average path velocity; Average path velocity, standard error; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Curvilinear velocity; Curvilinear velocity, standard error; EXP; Experiment; Fertilization success rate; Fluorescence intensity; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; Potentiometric; Potentiometric titration; Probability; Registration number of species; Replicate; Reproduction; Salinity; Salinity, standard error; Single species; Species; Straight line velocity; Straight line velocity, standard error; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference; Yueqing_Bay |
| url | https://doi.org/10.1594/PANGAEA.888184 |