Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2018
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| _version_ | 1867169127298236416 |
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| author | Cao, Ruiwen Wang, Qing Yang, Dinglong Liu, Yongliang Ran, Wen Qu, Yi Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin |
| author_facet | Cao, Ruiwen Wang, Qing Yang, Dinglong Liu, Yongliang Ran, Wen Qu, Yi Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a disturbed cytoskeleton structure, increased protein turnover and energy metabolism suppression, without causing oxidative stress. The combined OA- and V. splendidus-treated oysters ultimately presented a similar, but stronger proteomic response pattern compared with OA treatment alone. Overall, the impaired oyster immune functions caused by OA exposure may have increased the risk of V. splendidus infection. These results have important implications for the impact of OA on disease outbreaks in marine invertebrates, which would have significant economic and ecological repercussions. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_889437 |
| institution | PANGAEA |
| language | en |
| publishDate | 2018 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge Cao, Ruiwen Wang, Qing Yang, Dinglong Liu, Yongliang Ran, Wen Qu, Yi Wu, Huifeng Cong, Ming Li, Fei Ji, Chenglong Zhao, Jianmin Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Experiment duration; Fold change; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression, standard deviation; Group; Hemocyte count; Hemocyte count, standard deviation; Immunology/Self-protection; Laboratory experiment; Mollusca; Name; North Pacific; Number of expressed proteins; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH, NBS scale; pH, standard deviation; pH, total scale; Phagocytosis rate; Phagocytosis rate, standard deviation; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a disturbed cytoskeleton structure, increased protein turnover and energy metabolism suppression, without causing oxidative stress. The combined OA- and V. splendidus-treated oysters ultimately presented a similar, but stronger proteomic response pattern compared with OA treatment alone. Overall, the impaired oyster immune functions caused by OA exposure may have increased the risk of V. splendidus infection. These results have important implications for the impact of OA on disease outbreaks in marine invertebrates, which would have significant economic and ecological repercussions. |
| title | Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Experiment duration; Fold change; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression; Gene expression, standard deviation; Group; Hemocyte count; Hemocyte count, standard deviation; Immunology/Self-protection; Laboratory experiment; Mollusca; Name; North Pacific; Number of expressed proteins; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Percentage; Percentage, standard deviation; pH, NBS scale; pH, standard deviation; pH, total scale; Phagocytosis rate; Phagocytosis rate, standard deviation; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.889437 |