A biogeographical study of microbial substrate utilisation in the Atlantic Ocean
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| Auteurs principaux: | , , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2018
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| _version_ | 1867168194271117312 |
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| author | Reintjes, Greta Arnosti, Carol Fuchs, Bernhard M Amann, Rudolf |
| author_facet | Reintjes, Greta Arnosti, Carol Fuchs, Bernhard M Amann, Rudolf |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | A large fraction of the organic matter fixed in the oceans is transformed and remineralised by marine heterotrophic microorganisms. They, therefore, play a critical role in the marine carbon cycle. In this study, we set out to identify the roles played by individual heterotrophic bacteria in the degradation of high molecular weight polysaccharides. At five sites in the Atlantic Ocean, we investigated the processing of organic matter in microbial communities by tracking the changes in community composition (fluorescence in situ hybridisation (FISH), 16S rRNA tag sequencing) in substrate incubation using a defined concentration of a known fluorescently labelled polysaccharide (FLA-laminarin, FLA-xylan, and FLA-chondroitin sulfate). Additionally, we tracked the dynamics of substrate processing (selfish uptake and extracellular hydrolysis) within the microbial communities between sites. We found that the same substrate was processed in different ways by different members of a pelagic microbial community which points to significant follow-on effects for carbon cycling. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_892823 |
| institution | PANGAEA |
| language | en |
| publishDate | 2018 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | A biogeographical study of microbial substrate utilisation in the Atlantic Ocean Reintjes, Greta Arnosti, Carol Fuchs, Bernhard M Amann, Rudolf Abundance; Alteromonadales, substrate stained; Alteromonadales, targeted with ALT1413 oligonucleiotide FISH-Probe; AMT22-46; ATM22; ATM22_JC079_CTD-24; ATM22_JC079_CTD-38; ATM22_JC079_CTD-57; ATM22_JC079_CTD-8; Bacteroidetes, substrate stained; Bacteroidetes, targeted with CF319a oligonucliotide FISH-Probe; Catenovulum, substrate stained; Catenovulum, targets with CAT653 oligonucleotide FISH-Probe; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; Experiment day; Fluorescence in situ hybridization (FISH); James Cook; JC079; JC079-04; JC079-12; JC079-19; JC079-28; JC079-46; Latitude of event; Longitude of event; Microbial abundance, cells; Optional event label; Planctomycetes, substrate stained; Planctomycetes, targeted with PLA46 oligonucliotide FISH-Probe; Sample ID; Substrate type A large fraction of the organic matter fixed in the oceans is transformed and remineralised by marine heterotrophic microorganisms. They, therefore, play a critical role in the marine carbon cycle. In this study, we set out to identify the roles played by individual heterotrophic bacteria in the degradation of high molecular weight polysaccharides. At five sites in the Atlantic Ocean, we investigated the processing of organic matter in microbial communities by tracking the changes in community composition (fluorescence in situ hybridisation (FISH), 16S rRNA tag sequencing) in substrate incubation using a defined concentration of a known fluorescently labelled polysaccharide (FLA-laminarin, FLA-xylan, and FLA-chondroitin sulfate). Additionally, we tracked the dynamics of substrate processing (selfish uptake and extracellular hydrolysis) within the microbial communities between sites. We found that the same substrate was processed in different ways by different members of a pelagic microbial community which points to significant follow-on effects for carbon cycling. |
| title | A biogeographical study of microbial substrate utilisation in the Atlantic Ocean |
| topic | Abundance; Alteromonadales, substrate stained; Alteromonadales, targeted with ALT1413 oligonucleiotide FISH-Probe; AMT22-46; ATM22; ATM22_JC079_CTD-24; ATM22_JC079_CTD-38; ATM22_JC079_CTD-57; ATM22_JC079_CTD-8; Bacteroidetes, substrate stained; Bacteroidetes, targeted with CF319a oligonucliotide FISH-Probe; Catenovulum, substrate stained; Catenovulum, targets with CAT653 oligonucleotide FISH-Probe; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; Experiment day; Fluorescence in situ hybridization (FISH); James Cook; JC079; JC079-04; JC079-12; JC079-19; JC079-28; JC079-46; Latitude of event; Longitude of event; Microbial abundance, cells; Optional event label; Planctomycetes, substrate stained; Planctomycetes, targeted with PLA46 oligonucliotide FISH-Probe; Sample ID; Substrate type |
| url | https://doi.org/10.1594/PANGAEA.892823 |