Primary production using ¹⁴C method in subtropical gyres regions
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2019
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| author | Regaudie-de-Gioux, Aurore Huete-Ortega, Maria Sobrino, Cristina López-Sandoval, Daffne C González, Natalia Fernández-Carrera, Ana Vidal, Montserrat Marañón, Emilio Cermeño, Pedro Latasa, Mikel Agustí, Susana Duarte, Carlos |
| author_facet | Regaudie-de-Gioux, Aurore Huete-Ortega, Maria Sobrino, Cristina López-Sandoval, Daffne C González, Natalia Fernández-Carrera, Ana Vidal, Montserrat Marañón, Emilio Cermeño, Pedro Latasa, Mikel Agustí, Susana Duarte, Carlos |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Seawater was sampled from five depths in the euphotic zone corresponding to 100 % (ca. 3 m depth), 50 %, 20 %, 7 % and 1 % of incident Photosynthetically Active Radiation (PAR). For each depth, four 72 mL polystyrene bottles (three clear bottles and one dark bottle) were filled with unfiltered seawater, inoculated with 10 - 20 µCi NaH¹⁴CO₃ and incubated on-deck from dawn to dusk. Temperature and irradiance in the incubators simulated the water temperature and the incident irradiance at the corresponding depth of each sample by using a combination of neutral density and blue filters (Mist Blue, ref. 061, Lee Filters ®). After incubation, samples from three of the five depths (100 %, 20 % and 1 % PAR) were sequentially filtered through 20, 2 and 0.2 µm polycarbonate filters while the other depths (50 % and 7 % PAR) were directly filtered by 0.2 µm. Immediately after filtering, filters were then exposed to concentrated HCl fumes at least 12 h to remove the non-fixed inorganic ¹⁴C. Filters were placed in scintillation vials to which 5 mL of liquid scintillation cocktail was added. The radioactivity on each filter (disintegrations per minute, DPM) was determined using a Wallac scintillation counter. To compute the rate of photosynthetic carbon fixation, the dark-bottle DPM was subtracted from the light-bottle DPM values. A constant value of 24,720 µg L-1 (or 2,060 µmol L-1) was assumed for the concentration of dissolved inorganic carbon for surface waters in tropical ocean (Key et al., 2004). A correction factor of 1.05 was applied to this constant value for discrimination isotopic. Total primary production was calculated as the sum of the primary production on each size class. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_899853 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Primary production using ¹⁴C method in subtropical gyres regions Regaudie-de-Gioux, Aurore Huete-Ortega, Maria Sobrino, Cristina López-Sandoval, Daffne C González, Natalia Fernández-Carrera, Ana Vidal, Montserrat Marañón, Emilio Cermeño, Pedro Latasa, Mikel Agustí, Susana Duarte, Carlos 29HE20101215; 29HE20110117; 29HE20110211; 29HE20110317; 29HE20110416; 29HE20110513; 29HE20110619; CSIC; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; Hespérides; Latitude of event; Longitude of event; MALASPINA_LEG1; MALASPINA_LEG1_006-3; MALASPINA_LEG1_007-3; MALASPINA_LEG1_008-3; MALASPINA_LEG1_009-3; MALASPINA_LEG1_010-3; MALASPINA_LEG1_011-3; MALASPINA_LEG1_012-3; MALASPINA_LEG1_013-3; MALASPINA_LEG1_014-3; MALASPINA_LEG1_015-3; MALASPINA_LEG1_016-3; MALASPINA_LEG1_017-3; MALASPINA_LEG1_018-3; MALASPINA_LEG1_019-3; MALASPINA_LEG1_020-3; MALASPINA_LEG1_022-3; MALASPINA_LEG1_023-3; MALASPINA_LEG1_024-3; MALASPINA_LEG1_025-3; MALASPINA_LEG1_026-3; MALASPINA_LEG2; MALASPINA_LEG2_027-3; MALASPINA_LEG2_028-3; MALASPINA_LEG2_029-3; MALASPINA_LEG2_030-3; MALASPINA_LEG2_031-3; MALASPINA_LEG2_032-3; MALASPINA_LEG2_033-3; MALASPINA_LEG2_034-3; MALASPINA_LEG2_035-3; MALASPINA_LEG2_037-3; MALASPINA_LEG2_038-3; MALASPINA_LEG2_040-3; MALASPINA_LEG2_041-3; MALASPINA_LEG2_042-3; MALASPINA_LEG2_043-3; MALASPINA_LEG2_044-3; MALASPINA_LEG3; MALASPINA_LEG3_046-3; MALASPINA_LEG3_047-3; MALASPINA_LEG3_048-3; MALASPINA_LEG3_049-3; MALASPINA_LEG3_050-3; MALASPINA_LEG3_051-3; MALASPINA_LEG3_052-3; MALASPINA_LEG3_053-3; MALASPINA_LEG3_054-3; MALASPINA_LEG3_055-3; MALASPINA_LEG3_056-3; MALASPINA_LEG3_057-3; MALASPINA_LEG3_058-3; MALASPINA_LEG3_059-3; MALASPINA_LEG3_060-3; MALASPINA_LEG3_061-3; MALASPINA_LEG3_062-3; MALASPINA_LEG3_063-3; MALASPINA_LEG3_064-3; MALASPINA_LEG3_065-3; MALASPINA_LEG3_066-3; MALASPINA_LEG3_068-3; MALASPINA_LEG4; MALASPINA_LEG4_069-3; MALASPINA_LEG4_070-3; MALASPINA_LEG4_071-3; MALASPINA_LEG4_072-3; MALASPINA_LEG4_073-3; MALASPINA_LEG4_074-3; MALASPINA_LEG4_075-3; MALASPINA_LEG4_076-3; MALASPINA_LEG5; MALASPINA_LEG5_083-3; MALASPINA_LEG5_084-3; MALASPINA_LEG5_085-3; MALASPINA_LEG5_086-3; MALASPINA_LEG5_087-3; MALASPINA_LEG5_088-3; MALASPINA_LEG5_089-3; MALASPINA_LEG5_090-3; MALASPINA_LEG5_091-3; MALASPINA_LEG5_092-3; MALASPINA_LEG5_093-3; MALASPINA_LEG5_094-3; MALASPINA_LEG5_095-3; MALASPINA_LEG5_096-3; MALASPINA_LEG5_097-3; MALASPINA_LEG5_098-3; MALASPINA_LEG5_099-3; MALASPINA_LEG6; MALASPINA_LEG6_104-3; MALASPINA_LEG6_106-3; MALASPINA_LEG6_107-3; MALASPINA_LEG6_108-3; MALASPINA_LEG6_109-3; MALASPINA_LEG6_110-3; MALASPINA_LEG6_111-3; MALASPINA_LEG6_113-3; MALASPINA_LEG6_114-3; MALASPINA_LEG6_115-3; MALASPINA_LEG6_117-3; MALASPINA_LEG6_118-3; MALASPINA_LEG6_119-3; MALASPINA_LEG6_120-3; MALASPINA_LEG6_121-3; MALASPINA_LEG6_122-3; MALASPINA_LEG6_123-3; MALASPINA_LEG6_124-3; MALASPINA_LEG6_125-3; MALASPINA_LEG6_126-3; MALASPINA_LEG7; MALASPINA_LEG7_127-3; MALASPINA_LEG7_128-3; MALASPINA_LEG7_129-3; MALASPINA_LEG7_130-3; MALASPINA_LEG7_131-3; MALASPINA_LEG7_132-3; MALASPINA_LEG7_133-3; MALASPINA_LEG7_134-3; MALASPINA_LEG7_135-3; MALASPINA_LEG7_136-3; MALASPINA_LEG7_137-3; MALASPINA_LEG7_138-3; MALASPINA_LEG7_139-3; MALASPINA_LEG7_140-3; MALASPINA_LEG7_141-3; MALASPINA_LEG7_142-3; MALASPINA_LEG7_143-3; MALASPINA_LEG7_144-3; MALASPINA_LEG7_145-3; MALASPINA_LEG7_146-3; MALASPINA_LEG7_147-3; MALASPINA-2010; Malaspina circumnavigation expedition; MH008_006; MH009_007; MH010_008; MH011_009; MH012_010; MH013_011; MH014_012; MH015_013; MH016_014; MH017_015; MH018_016; MH019_017; MH020_018; MH021_019; MH022_020; MH024_022; MH025_023; MH026_024; MH027_025; MH028_026; MH036_027; MH037_028; MH038_029; MH039_030; MH040_031; MH041_032; MH042_033; MH043_034; MH044_035; MH046_037; MH047_038; MH049_040; MH050_041; MH051_042; MH052_043; MH053_044; MH062_046; MH063_047; MH064_048; MH065_049; MH066_050; MH067_051; MH072_052; MH073_053; MH074_054; MH075_055; MH076_056; MH077_057; MH078_058; MH079_059; MH080_060; MH081_061; MH082_062; MH083_063; MH084_064; MH085_065; MH086_066; MH088_068; MH095_069; MH096_070; MH097_071; MH098_072; MH099_073; MH100_074; MH101_075; MH102_076; MH127_083; MH128_084; MH129_085; MH130_086; MH131_087; MH132_088; MH133_089; MH134_090; MH135_091; MH136_092; MH137_093; MH138_094; MH139_095; MH140_096; MH141_097; MH142_098; MH143_099; MH153_104; MH155_106; MH156_107; MH157_108; MH158_109; MH159_110; MH160_111; MH162_113; MH163_114; MH164_115; MH166_117; MH167_118; MH168_119; MH169_120; MH170_121; MH171_122; MH172_123; MH173_124; MH174_125; MH175_126; MH188_127; MH189_128; MH190_129; MH191_130; MH193_131; MH194_132; MH195_133; MH196_134; MH197_135; MH198_136; MH199_137; MH200_138; MH201_139; MH202_140; MH203_141; MH204_142; MH205_143; MH206_144; MH207_145; MH208_146; MH209_147; Primary production; Primary production of carbon, standard deviation; Primary production of carbon per hour; see abstract; subtropical gyres Seawater was sampled from five depths in the euphotic zone corresponding to 100 % (ca. 3 m depth), 50 %, 20 %, 7 % and 1 % of incident Photosynthetically Active Radiation (PAR). For each depth, four 72 mL polystyrene bottles (three clear bottles and one dark bottle) were filled with unfiltered seawater, inoculated with 10 - 20 µCi NaH¹⁴CO₃ and incubated on-deck from dawn to dusk. Temperature and irradiance in the incubators simulated the water temperature and the incident irradiance at the corresponding depth of each sample by using a combination of neutral density and blue filters (Mist Blue, ref. 061, Lee Filters ®). After incubation, samples from three of the five depths (100 %, 20 % and 1 % PAR) were sequentially filtered through 20, 2 and 0.2 µm polycarbonate filters while the other depths (50 % and 7 % PAR) were directly filtered by 0.2 µm. Immediately after filtering, filters were then exposed to concentrated HCl fumes at least 12 h to remove the non-fixed inorganic ¹⁴C. Filters were placed in scintillation vials to which 5 mL of liquid scintillation cocktail was added. The radioactivity on each filter (disintegrations per minute, DPM) was determined using a Wallac scintillation counter. To compute the rate of photosynthetic carbon fixation, the dark-bottle DPM was subtracted from the light-bottle DPM values. A constant value of 24,720 µg L-1 (or 2,060 µmol L-1) was assumed for the concentration of dissolved inorganic carbon for surface waters in tropical ocean (Key et al., 2004). A correction factor of 1.05 was applied to this constant value for discrimination isotopic. Total primary production was calculated as the sum of the primary production on each size class. |
| title | Primary production using ¹⁴C method in subtropical gyres regions |
| topic | 29HE20101215; 29HE20110117; 29HE20110211; 29HE20110317; 29HE20110416; 29HE20110513; 29HE20110619; CSIC; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; Hespérides; Latitude of event; Longitude of event; MALASPINA_LEG1; MALASPINA_LEG1_006-3; MALASPINA_LEG1_007-3; MALASPINA_LEG1_008-3; MALASPINA_LEG1_009-3; MALASPINA_LEG1_010-3; MALASPINA_LEG1_011-3; MALASPINA_LEG1_012-3; MALASPINA_LEG1_013-3; MALASPINA_LEG1_014-3; MALASPINA_LEG1_015-3; MALASPINA_LEG1_016-3; MALASPINA_LEG1_017-3; MALASPINA_LEG1_018-3; MALASPINA_LEG1_019-3; MALASPINA_LEG1_020-3; MALASPINA_LEG1_022-3; MALASPINA_LEG1_023-3; MALASPINA_LEG1_024-3; MALASPINA_LEG1_025-3; MALASPINA_LEG1_026-3; MALASPINA_LEG2; MALASPINA_LEG2_027-3; MALASPINA_LEG2_028-3; MALASPINA_LEG2_029-3; MALASPINA_LEG2_030-3; MALASPINA_LEG2_031-3; MALASPINA_LEG2_032-3; MALASPINA_LEG2_033-3; MALASPINA_LEG2_034-3; MALASPINA_LEG2_035-3; MALASPINA_LEG2_037-3; MALASPINA_LEG2_038-3; MALASPINA_LEG2_040-3; MALASPINA_LEG2_041-3; MALASPINA_LEG2_042-3; MALASPINA_LEG2_043-3; MALASPINA_LEG2_044-3; MALASPINA_LEG3; MALASPINA_LEG3_046-3; MALASPINA_LEG3_047-3; MALASPINA_LEG3_048-3; MALASPINA_LEG3_049-3; MALASPINA_LEG3_050-3; MALASPINA_LEG3_051-3; MALASPINA_LEG3_052-3; MALASPINA_LEG3_053-3; MALASPINA_LEG3_054-3; MALASPINA_LEG3_055-3; MALASPINA_LEG3_056-3; MALASPINA_LEG3_057-3; MALASPINA_LEG3_058-3; MALASPINA_LEG3_059-3; MALASPINA_LEG3_060-3; MALASPINA_LEG3_061-3; MALASPINA_LEG3_062-3; MALASPINA_LEG3_063-3; MALASPINA_LEG3_064-3; MALASPINA_LEG3_065-3; MALASPINA_LEG3_066-3; MALASPINA_LEG3_068-3; MALASPINA_LEG4; MALASPINA_LEG4_069-3; MALASPINA_LEG4_070-3; MALASPINA_LEG4_071-3; MALASPINA_LEG4_072-3; MALASPINA_LEG4_073-3; MALASPINA_LEG4_074-3; MALASPINA_LEG4_075-3; MALASPINA_LEG4_076-3; MALASPINA_LEG5; MALASPINA_LEG5_083-3; MALASPINA_LEG5_084-3; MALASPINA_LEG5_085-3; MALASPINA_LEG5_086-3; MALASPINA_LEG5_087-3; MALASPINA_LEG5_088-3; MALASPINA_LEG5_089-3; MALASPINA_LEG5_090-3; MALASPINA_LEG5_091-3; MALASPINA_LEG5_092-3; MALASPINA_LEG5_093-3; MALASPINA_LEG5_094-3; MALASPINA_LEG5_095-3; MALASPINA_LEG5_096-3; MALASPINA_LEG5_097-3; MALASPINA_LEG5_098-3; MALASPINA_LEG5_099-3; MALASPINA_LEG6; MALASPINA_LEG6_104-3; MALASPINA_LEG6_106-3; MALASPINA_LEG6_107-3; MALASPINA_LEG6_108-3; MALASPINA_LEG6_109-3; MALASPINA_LEG6_110-3; MALASPINA_LEG6_111-3; MALASPINA_LEG6_113-3; MALASPINA_LEG6_114-3; MALASPINA_LEG6_115-3; MALASPINA_LEG6_117-3; MALASPINA_LEG6_118-3; MALASPINA_LEG6_119-3; MALASPINA_LEG6_120-3; MALASPINA_LEG6_121-3; MALASPINA_LEG6_122-3; MALASPINA_LEG6_123-3; MALASPINA_LEG6_124-3; MALASPINA_LEG6_125-3; MALASPINA_LEG6_126-3; MALASPINA_LEG7; MALASPINA_LEG7_127-3; MALASPINA_LEG7_128-3; MALASPINA_LEG7_129-3; MALASPINA_LEG7_130-3; MALASPINA_LEG7_131-3; MALASPINA_LEG7_132-3; MALASPINA_LEG7_133-3; MALASPINA_LEG7_134-3; MALASPINA_LEG7_135-3; MALASPINA_LEG7_136-3; MALASPINA_LEG7_137-3; MALASPINA_LEG7_138-3; MALASPINA_LEG7_139-3; MALASPINA_LEG7_140-3; MALASPINA_LEG7_141-3; MALASPINA_LEG7_142-3; MALASPINA_LEG7_143-3; MALASPINA_LEG7_144-3; MALASPINA_LEG7_145-3; MALASPINA_LEG7_146-3; MALASPINA_LEG7_147-3; MALASPINA-2010; Malaspina circumnavigation expedition; MH008_006; MH009_007; MH010_008; MH011_009; MH012_010; MH013_011; MH014_012; MH015_013; MH016_014; MH017_015; MH018_016; MH019_017; MH020_018; MH021_019; MH022_020; MH024_022; MH025_023; MH026_024; MH027_025; MH028_026; MH036_027; MH037_028; MH038_029; MH039_030; MH040_031; MH041_032; MH042_033; MH043_034; MH044_035; MH046_037; MH047_038; MH049_040; MH050_041; MH051_042; MH052_043; MH053_044; MH062_046; MH063_047; MH064_048; MH065_049; MH066_050; MH067_051; MH072_052; MH073_053; MH074_054; MH075_055; MH076_056; MH077_057; MH078_058; MH079_059; MH080_060; MH081_061; MH082_062; MH083_063; MH084_064; MH085_065; MH086_066; MH088_068; MH095_069; MH096_070; MH097_071; MH098_072; MH099_073; MH100_074; MH101_075; MH102_076; MH127_083; MH128_084; MH129_085; MH130_086; MH131_087; MH132_088; MH133_089; MH134_090; MH135_091; MH136_092; MH137_093; MH138_094; MH139_095; MH140_096; MH141_097; MH142_098; MH143_099; MH153_104; MH155_106; MH156_107; MH157_108; MH158_109; MH159_110; MH160_111; MH162_113; MH163_114; MH164_115; MH166_117; MH167_118; MH168_119; MH169_120; MH170_121; MH171_122; MH172_123; MH173_124; MH174_125; MH175_126; MH188_127; MH189_128; MH190_129; MH191_130; MH193_131; MH194_132; MH195_133; MH196_134; MH197_135; MH198_136; MH199_137; MH200_138; MH201_139; MH202_140; MH203_141; MH204_142; MH205_143; MH206_144; MH207_145; MH208_146; MH209_147; Primary production; Primary production of carbon, standard deviation; Primary production of carbon per hour; see abstract; subtropical gyres |
| url | https://doi.org/10.1594/PANGAEA.899853 |