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| Autori principali: | , , , , |
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| Natura: | Dataset Open Access |
| Lingua: | en |
| Pubblicazione: |
PANGAEA
2019
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| Soggetti: | |
| Accesso online: | https://doi.org/10.1594/PANGAEA.904040 |
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| _version_ | 1867169134094057472 |
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| author | Hanke, Inken Hassenrück, Christiane Kunzmann, Andreas Aerts, Johan Gärdes, Astrid |
| author_facet | Hanke, Inken Hassenrück, Christiane Kunzmann, Andreas Aerts, Johan Gärdes, Astrid |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | To quantify chronic stress levels in milkfish cultured within a typical commercial mariculture setting, we measured ontogenetic (OG) scale cortisol of milkfish sampled from four cage systems (F1-F4) with different geographic locations and management strategies. Sampling stations were located within a mariculture area (approx. 2.2 x 0.6 km) of the Guiguiwanen Channel, Bolinao (Philippines). Each station was sampled twice (time point I and II) with an intermediate time interval of 15 days. According to Aerts et al. (2015), ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was used to analyze OG scale cortisol. Additional to the stress-physiological data, environmental parameters (oxygen concentration, salinity) were recorded, and the gut content of the fish and the fish feed were analyzed for their carbon (C) and nitrogen (N) content, CN stable isotope signatures, presence of antibiotics (LC-MS/MS), organic matter (pyrolysis-GCMS, MetaboLights accession MTBLS1438), microbial community composition (16S amplicon sequencing, PRJEB33594, OTU table available here), and abundance of the virulence factor gene thermolabile hemolysin of Vibrio sp. (qPCR, primers: tlh-G-vibrio-0515-a-S-22: 5'-GCTGGTTCTTRGGDCAYTTCTC-3', tlh-G-vibrio-0750-a-A-22: 5'-TGGAACGCYACGGTTRTAGTTC-3'). Furthermore, the sampling took place as part of a larger sampling campaign within the ACUTE project (AquaCUlture practice in Tropical coastal Ecosystems: Understanding ecological and socio-economic consequences; funding: Leibniz SAW-2015-ZMT-4), during which also sediment microbial communities and environmental parameters were surveyed (Moncada et al. (2019) doi:10.1093/femsec/fiz006, NCBI Sequence Read Archive (SRA) accession: SRP155505, doi:10.1594/PANGAEA.900758). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_904040 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Ontogenetic scale cortisol, body condition, and gut content analyses of juvenile milkfish (Chanos chanos) cultured in commercial marine cage systems Hanke, Inken Hassenrück, Christiane Kunzmann, Andreas Aerts, Johan Gärdes, Astrid after Aerts et al. (2015); Calculated; Carbon, organic, total; Carbon/Nitrogen ratio; Condition factor; Cortisol; DATE/TIME; DEPTH, water; Description; Elemental analyzer, EuroVector, EA 3000; Event label; Fish; Fluorescence-based quantitative real-time PCR (qPCR); Gene copies; Guiguiwanen_channel-F1; Guiguiwanen_channel-F2; Guiguiwanen_channel-F3; Guiguiwanen_channel-F4; Host; Length; Liver, mass; marine aquaculture; Mass; microbial communities; Nitrogen, total; Number; Philippines; Sample code/label; Sample comment; Sample ID; Size; Station label; Time in days; Time point, descriptive; Weighed (scale); δ13C, organic carbon; δ15N, total dissolved nitrogen To quantify chronic stress levels in milkfish cultured within a typical commercial mariculture setting, we measured ontogenetic (OG) scale cortisol of milkfish sampled from four cage systems (F1-F4) with different geographic locations and management strategies. Sampling stations were located within a mariculture area (approx. 2.2 x 0.6 km) of the Guiguiwanen Channel, Bolinao (Philippines). Each station was sampled twice (time point I and II) with an intermediate time interval of 15 days. According to Aerts et al. (2015), ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) was used to analyze OG scale cortisol. Additional to the stress-physiological data, environmental parameters (oxygen concentration, salinity) were recorded, and the gut content of the fish and the fish feed were analyzed for their carbon (C) and nitrogen (N) content, CN stable isotope signatures, presence of antibiotics (LC-MS/MS), organic matter (pyrolysis-GCMS, MetaboLights accession MTBLS1438), microbial community composition (16S amplicon sequencing, PRJEB33594, OTU table available here), and abundance of the virulence factor gene thermolabile hemolysin of Vibrio sp. (qPCR, primers: tlh-G-vibrio-0515-a-S-22: 5'-GCTGGTTCTTRGGDCAYTTCTC-3', tlh-G-vibrio-0750-a-A-22: 5'-TGGAACGCYACGGTTRTAGTTC-3'). Furthermore, the sampling took place as part of a larger sampling campaign within the ACUTE project (AquaCUlture practice in Tropical coastal Ecosystems: Understanding ecological and socio-economic consequences; funding: Leibniz SAW-2015-ZMT-4), during which also sediment microbial communities and environmental parameters were surveyed (Moncada et al. (2019) doi:10.1093/femsec/fiz006, NCBI Sequence Read Archive (SRA) accession: SRP155505, doi:10.1594/PANGAEA.900758). |
| title | Ontogenetic scale cortisol, body condition, and gut content analyses of juvenile milkfish (Chanos chanos) cultured in commercial marine cage systems |
| topic | after Aerts et al. (2015); Calculated; Carbon, organic, total; Carbon/Nitrogen ratio; Condition factor; Cortisol; DATE/TIME; DEPTH, water; Description; Elemental analyzer, EuroVector, EA 3000; Event label; Fish; Fluorescence-based quantitative real-time PCR (qPCR); Gene copies; Guiguiwanen_channel-F1; Guiguiwanen_channel-F2; Guiguiwanen_channel-F3; Guiguiwanen_channel-F4; Host; Length; Liver, mass; marine aquaculture; Mass; microbial communities; Nitrogen, total; Number; Philippines; Sample code/label; Sample comment; Sample ID; Size; Station label; Time in days; Time point, descriptive; Weighed (scale); δ13C, organic carbon; δ15N, total dissolved nitrogen |
| url | https://doi.org/10.1594/PANGAEA.904040 |