Experiment on phage-bacteria infection networks at ambient (15 PSU) and two stressful salinities (11 & 7 PSU) with Vibrio alginolyticus
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2019
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| _version_ | 1867170474596761600 |
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| author | Goehlich, Henry Roth, Olivia Wendling, Carolin Charlotte |
| author_facet | Goehlich, Henry Roth, Olivia Wendling, Carolin Charlotte |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Being non-lytic, filamentous phages can replicate at high frequencies and often carry virulence factors, which are important in the evolution and emergence of novel pathogens. However, their net-effect on bacterial fitness remains unknown. To understand the ecology and evolution between filamentous phages and their hosts, it is important to understand (1) fitness effects of a filamentous phages on their hosts, and (2) how these effects depend on the environment. To determine how the net-effect on bacterial fitness by filamentous phages changes across environments, we constructed phage-bacteria infection networks at ambient (15 PSU) and two stressful salinities (11 & 7 PSU) using the marine bacterium, Vibrio alginolyticus and its derived filamentous phages as model system. We observed no significant difference in network structure at 15 PSU and 11 PSU. However, at 7 PSU phages significantly reduced bacterial growth changing network structure. This pattern was mainly driven by a significant increase in bacterial susceptibility. Our findings suggest that filamentous phages decrease bacterial fitness at stress-full environmental conditions, which may impact bacterial communities, alter horizontal gene transfer events and favour the evolution of novel pathogens in environmental Vibrios. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_906121 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Experiment on phage-bacteria infection networks at ambient (15 PSU) and two stressful salinities (11 & 7 PSU) with Vibrio alginolyticus Goehlich, Henry Roth, Olivia Wendling, Carolin Charlotte Filamentous phages; growth; Phage-bacteria network; Salinity; Vibrio alginolyticus Being non-lytic, filamentous phages can replicate at high frequencies and often carry virulence factors, which are important in the evolution and emergence of novel pathogens. However, their net-effect on bacterial fitness remains unknown. To understand the ecology and evolution between filamentous phages and their hosts, it is important to understand (1) fitness effects of a filamentous phages on their hosts, and (2) how these effects depend on the environment. To determine how the net-effect on bacterial fitness by filamentous phages changes across environments, we constructed phage-bacteria infection networks at ambient (15 PSU) and two stressful salinities (11 & 7 PSU) using the marine bacterium, Vibrio alginolyticus and its derived filamentous phages as model system. We observed no significant difference in network structure at 15 PSU and 11 PSU. However, at 7 PSU phages significantly reduced bacterial growth changing network structure. This pattern was mainly driven by a significant increase in bacterial susceptibility. Our findings suggest that filamentous phages decrease bacterial fitness at stress-full environmental conditions, which may impact bacterial communities, alter horizontal gene transfer events and favour the evolution of novel pathogens in environmental Vibrios. |
| title | Experiment on phage-bacteria infection networks at ambient (15 PSU) and two stressful salinities (11 & 7 PSU) with Vibrio alginolyticus |
| topic | Filamentous phages; growth; Phage-bacteria network; Salinity; Vibrio alginolyticus |
| url | https://doi.org/10.1594/PANGAEA.906121 |