Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2019
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| _version_ | 1867170095210430464 |
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| author | Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun |
| author_facet | Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This work demonstrated a 10-day batch culture experiment to test the physiology and toxicity of harmful dinoflagellate Karenia mikimotoi in response to ocean acidification (OA) under two different phosphate concentrations. Cells were previously acclimated in OA (pH = 7.8 and CO2 = 1100 μatm) condition for about three months before testing the responses of K. mikimotoi cells to a two-factorial combinations experimentation. This work measured the variation in physiological parameters (growth, rETR) and toxicity (hemolytic activity and its toxicity to zebrafish embryos) in four treatments, representing two factorial combinations of CO2 (450 and 1100 μatm) and phosphate concentration (37.75 and 4.67 umol l−1). Results: OA stimulated the faster growth, and the highest rETRmax in high phosphate (HP) treatment, low phosphate (LP) and a combination of high CO2 and low phosphate (HC*LP) inhibited the growth and Ek in comparison to low CO2*high phosphate (LCHP) treatment. The embryotoxicity of K. mikimotoi cells enhanced in all high CO2 (HC) conditions irrespective of phosphate concentration, but the EC50 of hemolytic activity increased in all high CO2 (HC) and low phosphate (LP) treatments in comparison of LCHP. Ocean acidification (high CO2 and lower pH) was probably the main factor that affected the rETRmax, hemolytic activity and embryotoxicity, but low phosphate was the main factor that affected the growth, α, and Ek. There were significant interactive effects of OA and low phosphate (LP) on growth, rETRmax, and hemolytic activity, but there were no significant effects on α, Ek, and embryotoxicity. If these results are extrapolated to the aquatic environment, it can be hypothesized that the K. mikimotoi cells were impacted significantly by future changing ocean (e.g., ocean acidification and nutrient stoichiometry). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_907178 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi Wang, Hong Niu, Xiaoqin Feng, Xinqian Gonçalves, Rodrigo J Guan, WanChun Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Cell density; Cell density, standard deviation; Chromista; Deformation rate; Deformation rate, standard deviation; Electron transport rate, relative; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haemolytic activity; Haemolytic activity, standard deviation; Identification; Immunology/Self-protection; Irradiance; Karenia mikimotoi; Laboratory experiment; Macro-nutrients; Myzozoa; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Primary production/Photosynthesis; Registration number of species; Salinity; Single species; Species; Temperature, water; Time in hours; Treatment; Type; Uniform resource locator/link to reference This work demonstrated a 10-day batch culture experiment to test the physiology and toxicity of harmful dinoflagellate Karenia mikimotoi in response to ocean acidification (OA) under two different phosphate concentrations. Cells were previously acclimated in OA (pH = 7.8 and CO2 = 1100 μatm) condition for about three months before testing the responses of K. mikimotoi cells to a two-factorial combinations experimentation. This work measured the variation in physiological parameters (growth, rETR) and toxicity (hemolytic activity and its toxicity to zebrafish embryos) in four treatments, representing two factorial combinations of CO2 (450 and 1100 μatm) and phosphate concentration (37.75 and 4.67 umol l−1). Results: OA stimulated the faster growth, and the highest rETRmax in high phosphate (HP) treatment, low phosphate (LP) and a combination of high CO2 and low phosphate (HC*LP) inhibited the growth and Ek in comparison to low CO2*high phosphate (LCHP) treatment. The embryotoxicity of K. mikimotoi cells enhanced in all high CO2 (HC) conditions irrespective of phosphate concentration, but the EC50 of hemolytic activity increased in all high CO2 (HC) and low phosphate (LP) treatments in comparison of LCHP. Ocean acidification (high CO2 and lower pH) was probably the main factor that affected the rETRmax, hemolytic activity and embryotoxicity, but low phosphate was the main factor that affected the growth, α, and Ek. There were significant interactive effects of OA and low phosphate (LP) on growth, rETRmax, and hemolytic activity, but there were no significant effects on α, Ek, and embryotoxicity. If these results are extrapolated to the aquatic environment, it can be hypothesized that the K. mikimotoi cells were impacted significantly by future changing ocean (e.g., ocean acidification and nutrient stoichiometry). |
| title | Seawater carbonate chemistry and physiology and toxicity of the dinoflagellate Karenia mikimotoi |
| topic | Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Cell density; Cell density, standard deviation; Chromista; Deformation rate; Deformation rate, standard deviation; Electron transport rate, relative; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haemolytic activity; Haemolytic activity, standard deviation; Identification; Immunology/Self-protection; Irradiance; Karenia mikimotoi; Laboratory experiment; Macro-nutrients; Myzozoa; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Primary production/Photosynthesis; Registration number of species; Salinity; Single species; Species; Temperature, water; Time in hours; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.907178 |