Seawater carbonate chemistry and metabolic rate, ventilation rate and critical oxygen partial pressure of Dosidicus gigas and Doryteuthis pealeii

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Main Authors: Birk, Matthew A, McLean, Erin L, Seibel, Brad A
Format: Dataset Open Access
Language:en
Published: PANGAEA 2018
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_version_ 1867170096319823872
author Birk, Matthew A
McLean, Erin L
Seibel, Brad A
author_facet Birk, Matthew A
McLean, Erin L
Seibel, Brad A
collection Datos científicos de ciencias marinas y ambientales
contents Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in Pcrit. In this study, we assessed the effects of CO2 (46–143 Pa; 455–1410 μatm) on the metabolic rate and Pcrit of two squid species - Dosidicus gigas and Doryteuthis pealeii – through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood PCO2 and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured Pcrit in squid. Using blood–O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid–base regulation, an increase in seawater PCO2 to 100 Pa (1000 μatm) would result in a maximum drop in arterial hemocyanin–O2 saturation by 1.6% at normoxia and a Pcrit increase of 0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or Pcrit in either squid species.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_909481
institution PANGAEA
language en
publishDate 2018
publisher PANGAEA
record_format pangaea
spellingShingle Seawater carbonate chemistry and metabolic rate, ventilation rate and critical oxygen partial pressure of Dosidicus gigas and Doryteuthis pealeii
Birk, Matthew A
McLean, Erin L
Seibel, Brad A
Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Behaviour; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Doryteuthis pealeii; Dosidicus gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gender; Growth/Morphology; Identification; Laboratory experiment; Mantle, length; Mass; Metabolic rate of oxygen; Mollusca; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Oxygen, partial pressure; Oxygen, partial pressure, critical; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, total scale; Registration number of species; Respiration; Salinity; Single species; Species; Temperate; Temperature, water; Type; Uniform resource locator/link to reference; Ventilation rate
Ocean acidification is hypothesized to limit the performance of squid owing to their exceptional oxygen demand and pH sensitivity of blood–oxygen binding, which may reduce oxygen supply in acidified waters. The critical oxygen partial pressure (Pcrit), the PO2 below which oxygen supply cannot match basal demand, is a commonly reported index of hypoxia tolerance. Any CO2-induced reduction in oxygen supply should be apparent as an increase in Pcrit. In this study, we assessed the effects of CO2 (46–143 Pa; 455–1410 μatm) on the metabolic rate and Pcrit of two squid species - Dosidicus gigas and Doryteuthis pealeii – through manipulative experiments. We also developed a model, with inputs for hemocyanin pH sensitivity, blood PCO2 and buffering capacity, that simulates blood oxygen supply under varying seawater CO2 partial pressures. We compare model outputs with measured Pcrit in squid. Using blood–O2 parameters from the literature for model inputs, we estimated that, in the absence of blood acid–base regulation, an increase in seawater PCO2 to 100 Pa (1000 μatm) would result in a maximum drop in arterial hemocyanin–O2 saturation by 1.6% at normoxia and a Pcrit increase of 0.5 kPa. Our live-animal experiments support this supposition, as CO2 had no effect on measured metabolic rate or Pcrit in either squid species.
title Seawater carbonate chemistry and metabolic rate, ventilation rate and critical oxygen partial pressure of Dosidicus gigas and Doryteuthis pealeii
topic Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Behaviour; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Doryteuthis pealeii; Dosidicus gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gender; Growth/Morphology; Identification; Laboratory experiment; Mantle, length; Mass; Metabolic rate of oxygen; Mollusca; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Oxygen, partial pressure; Oxygen, partial pressure, critical; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, total scale; Registration number of species; Respiration; Salinity; Single species; Species; Temperate; Temperature, water; Type; Uniform resource locator/link to reference; Ventilation rate
url https://doi.org/10.1594/PANGAEA.909481