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| Main Authors: | , , , , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2020
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.913421 |
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| _version_ | 1867168488785707008 |
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| author | Weber, Judith Sophie Shaida, Stephen Saikiu Ngomtcho, Sen Claudine Henriette Musa, Usman Baba Adamu, Ahmadu Gbem, Thaddeus Terlumun Berger, Petra Mamman, Mohammed Nok, Jonathan Andrew Kelm, Sørge |
| author_facet | Weber, Judith Sophie Shaida, Stephen Saikiu Ngomtcho, Sen Claudine Henriette Musa, Usman Baba Adamu, Ahmadu Gbem, Thaddeus Terlumun Berger, Petra Mamman, Mohammed Nok, Jonathan Andrew Kelm, Sørge |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This data set includes the results of nested PCR-analysis of tsetse (Glossina sp.) gut samples with primers described by Adams et.al. (2006) and Hamilton et al (2004) from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. The gut samples were homogenized in the field. DNA was purified from preserved homogenized gut using the Qiagen Blood and Tissue Kit. Presence of trypanosomes was investigated by amplification of Internal Transcribed Spacer-1 (ITS1). Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region by estimating the approximate size of the DNA amplicon based on electrophoretic separation in agarose gels, as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_913421 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Tryapanosoma prevalence in tsetse (Glossina sp.) gut collected in protected and tsetse invested areas in Nigeria Weber, Judith Sophie Shaida, Stephen Saikiu Ngomtcho, Sen Claudine Henriette Musa, Usman Baba Adamu, Ahmadu Gbem, Thaddeus Terlumun Berger, Petra Mamman, Mohammed Nok, Jonathan Andrew Kelm, Sørge Animal African Trypanosomiasis in humans: An emerging zoonosis?; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; TRYPTS; TRYPZOO This data set includes the results of nested PCR-analysis of tsetse (Glossina sp.) gut samples with primers described by Adams et.al. (2006) and Hamilton et al (2004) from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. The gut samples were homogenized in the field. DNA was purified from preserved homogenized gut using the Qiagen Blood and Tissue Kit. Presence of trypanosomes was investigated by amplification of Internal Transcribed Spacer-1 (ITS1). Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region by estimating the approximate size of the DNA amplicon based on electrophoretic separation in agarose gels, as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase. |
| title | Tryapanosoma prevalence in tsetse (Glossina sp.) gut collected in protected and tsetse invested areas in Nigeria |
| topic | Animal African Trypanosomiasis in humans: An emerging zoonosis?; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; TRYPTS; TRYPZOO |
| url | https://doi.org/10.1594/PANGAEA.913421 |