Seawater carbonate chemistry and biochemical composition of the coccolithophore Emiliania huxleyi
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2019
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| _version_ | 1867171867223130112 |
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| author | Heidenreich, Elena Wördenweber, Robin Kirschhöfer, Frank Nusser, Michael Friedrich, Frank Fahl, Kirsten Kruse, Olaf Rost, Björn Franzreb, Matthias Brenner-Weiß, Gerald Rokitta, Sebastian D |
| author_facet | Heidenreich, Elena Wördenweber, Robin Kirschhöfer, Frank Nusser, Michael Friedrich, Frank Fahl, Kirsten Kruse, Olaf Rost, Björn Franzreb, Matthias Brenner-Weiß, Gerald Rokitta, Sebastian D |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi's biomass composition. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_913444 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and biochemical composition of the coccolithophore Emiliania huxleyi Heidenreich, Elena Wördenweber, Robin Kirschhöfer, Frank Nusser, Michael Friedrich, Frank Fahl, Kirsten Kruse, Olaf Rost, Björn Franzreb, Matthias Brenner-Weiß, Gerald Rokitta, Sebastian D Alkalinity, total; Alkalinity, total, standard deviation; Alkenones; Alkenones, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Category; Cell, diameter; Cell, diameter, standard deviation; Chromista; Concentration; Concentration, standard deviation; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haptophyta; Laboratory experiment; Laboratory strains; Light; Name; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Registration number of species; Salinity; Single species; Species; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi's biomass composition. |
| title | Seawater carbonate chemistry and biochemical composition of the coccolithophore Emiliania huxleyi |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Alkenones; Alkenones, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Category; Cell, diameter; Cell, diameter, standard deviation; Chromista; Concentration; Concentration, standard deviation; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haptophyta; Laboratory experiment; Laboratory strains; Light; Name; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Registration number of species; Salinity; Single species; Species; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.913444 |