Exp. I: cercarial emergence from periwinkles
Fuente:
PANGAEA
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Dataset Open Access |
| Sprache: | en |
| Veröffentlicht: |
PANGAEA
2020
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1867172404716896256 |
|---|---|
| author | Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W |
| author_facet | Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Cercarial emergence was assessed by counting all cercariae that emerged from single periwinkles exposed to the different treatments. Periwinkles were collected in Årøsund, Denmark and screened in the laboratory to verify the infection status. To measure cercarial emergence, periwinkles were individually transferred from the jars into 50 mL Plexiglas beakers, one day after the 7-days acclimation (sampling 1), one week after the start of exposure to the treatments (sampling 2), and two weeks after the start of exposure to the treatments (sampling 3). Each incubation lasted eight hours. At the end of the incubation period, the water from each beaker was transferred into 50 mL Falcon tubes. In addition, the beakers were immediately washed with 5 mL ethanol (99%), and the solution was also added to the Falcon tube to ensure the collection of all cercariae and their preservation for later counting. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_914092 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Exp. I: cercarial emergence from periwinkles Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W Himasthla elongata, per infected host individual; Identification; infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; Sampling; Species; Treatment; Treatment: temperature; trematode Cercarial emergence was assessed by counting all cercariae that emerged from single periwinkles exposed to the different treatments. Periwinkles were collected in Årøsund, Denmark and screened in the laboratory to verify the infection status. To measure cercarial emergence, periwinkles were individually transferred from the jars into 50 mL Plexiglas beakers, one day after the 7-days acclimation (sampling 1), one week after the start of exposure to the treatments (sampling 2), and two weeks after the start of exposure to the treatments (sampling 3). Each incubation lasted eight hours. At the end of the incubation period, the water from each beaker was transferred into 50 mL Falcon tubes. In addition, the beakers were immediately washed with 5 mL ethanol (99%), and the solution was also added to the Falcon tube to ensure the collection of all cercariae and their preservation for later counting. |
| title | Exp. I: cercarial emergence from periwinkles |
| topic | Himasthla elongata, per infected host individual; Identification; infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; Sampling; Species; Treatment; Treatment: temperature; trematode |
| url | https://doi.org/10.1594/PANGAEA.914092 |