Exp. II: cercarial activity and survival
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| Autores principales: | , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2020
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| _version_ | 1867170381929906176 |
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| author | Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W |
| author_facet | Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | For cercarial activity and survival infected periwinkles were acclimated for three weeks under the different treatments (n=10 individuals per treatment) and incubated to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment were added individually to wells of three 96-well plates The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three 96-well plates per treatment combination). Activity and survival were then assessed under a stereomicroscope after 4, 6, 9, 18, 27, and 45 hours. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When laying at the bottom and not reacting, these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included "fully active". |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_914100 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Exp. II: cercarial activity and survival Bommarito, Claudia Pansch, Christian Khosravi, Maral Pranovi, Fabio Wahl, Martin Thieltges, David W Identification; infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; Species; Status; Time in hours; Treatment; Treatment: temperature; trematode; Variable For cercarial activity and survival infected periwinkles were acclimated for three weeks under the different treatments (n=10 individuals per treatment) and incubated to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment were added individually to wells of three 96-well plates The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three 96-well plates per treatment combination). Activity and survival were then assessed under a stereomicroscope after 4, 6, 9, 18, 27, and 45 hours. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When laying at the bottom and not reacting, these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included "fully active". |
| title | Exp. II: cercarial activity and survival |
| topic | Identification; infectivity; Littorina littorea; Mytilus galloprovincialis; Salinity; Species; Status; Time in hours; Treatment; Treatment: temperature; trematode; Variable |
| url | https://doi.org/10.1594/PANGAEA.914100 |