Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima)
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2019
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| _version_ | 1867169140543848448 |
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| author | Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben |
| author_facet | Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_920153 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) Hermann, Bernd Timo Würtz, Sven Vanselow, Klaus Heinrich Schulz, Carsten Stiller, Kevin Torben Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; CNRQs, Quantitative reverse transcription PCR; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gill, general hyperplasia; Gill, hyperplasia on basis of primary filament; Gill, hyperplasia on distal part of secondary lamellae; Gill, hypertrophied chloride cells; Gill, hypertrophy of secondary lamellae; Gill, lamellar clubbing; Gill, not affected; Laboratory experiment; Microscopy; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Psetta maxima; Quantitative reverse transcription polymerase chain reaction (qRT-PCR); Registration number of species; Replicate; Salinity; Sample ID; Sample type; Single species; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm. |
| title | Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima) |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chordata; CNRQs, Quantitative reverse transcription PCR; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Gill, general hyperplasia; Gill, hyperplasia on basis of primary filament; Gill, hyperplasia on distal part of secondary lamellae; Gill, hypertrophied chloride cells; Gill, hypertrophy of secondary lamellae; Gill, lamellar clubbing; Gill, not affected; Laboratory experiment; Microscopy; Nekton; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Psetta maxima; Quantitative reverse transcription polymerase chain reaction (qRT-PCR); Registration number of species; Replicate; Salinity; Sample ID; Sample type; Single species; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.920153 |