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Main Authors: Griffiths, Joanna S, Pan, Tien-Chien Francis, Kelly, Morgan W
Format: Dataset Open Access
Language:en
Published: PANGAEA 2019
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Online Access:https://doi.org/10.1594/PANGAEA.920209
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author Griffiths, Joanna S
Pan, Tien-Chien Francis
Kelly, Morgan W
author_facet Griffiths, Joanna S
Pan, Tien-Chien Francis
Kelly, Morgan W
collection Datos científicos de ciencias marinas y ambientales
contents Ocean acidification (OA), the global decrease in surface water pH from absorption of anthropogenic CO2, may put many marine taxa at risk. However, populations that experience extreme localized conditions, and are adapted to these conditions predicted in the global ocean in 2100, may be more tolerant to future OA. By identifying locally adapted populations, researchers can examine the mechanisms used to cope with decreasing pH. One oceanographic process that influences pH, is wind driven upwelling. Here we compare two Californian populations of the coral Balanophyllia elegans from distinct upwelling regimes, and test their physiological and transcriptomic responses to experimental seawater acidification. We measured respiration rates, protein and lipid content, and gene expression in corals from both populations exposed to pH levels of 7.8 and 7.4 for 29 days. Corals from the population that experiences lower pH due to high upwelling, maintained the same respiration rate throughout the exposure. In contrast, corals from the low upwelling site had reduced respiration rates, protein content, and lipid‐class content at low pH exposure, suggesting they have depleted their energy reserves. Using RNA‐Seq, we found that corals from the high upwelling site upregulated genes involved in calcium ion binding and ion transport, most likely related to pH homeostasis and calcification. In contrast, corals from the low upwelling site downregulated stress response genes at low pH exposure. Divergent population responses to low pH observed in B. elegans highlight the importance of multi‐population studies for predicting a species' response to future OA.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_920209
institution PANGAEA
language en
publishDate 2019
publisher PANGAEA
record_format pangaea
spellingShingle Seawater carbonate chemistry and respiration rates, protein and lipid content of Balanophyllia elegans
Griffiths, Joanna S
Pan, Tien-Chien Francis
Kelly, Morgan W
Alkalinity, total; Animalia; Aragonite saturation state; Balanophyllia elegans; Benthic animals; Benthos; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; Dry mass; Event label; EXP; Experiment; Experiment duration; Fatty acids, total; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Globigerinatheka cf. index; Goleta; Identification; Incubation duration; Laboratory experiment; Lipids, total; Location; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, total scale; Phospholipid fatty acids; Point_Arena_Cove; Potentiometric; Potentiometric titration; Proteins, total; Registration number of species; Respiration; Respiration rate, oxygen; Salinity; Single species; Species; Sterols; Temperate; Temperature, water; Treatment; Triacylglycerols, per dry mass; Type; Uniform resource locator/link to reference
Ocean acidification (OA), the global decrease in surface water pH from absorption of anthropogenic CO2, may put many marine taxa at risk. However, populations that experience extreme localized conditions, and are adapted to these conditions predicted in the global ocean in 2100, may be more tolerant to future OA. By identifying locally adapted populations, researchers can examine the mechanisms used to cope with decreasing pH. One oceanographic process that influences pH, is wind driven upwelling. Here we compare two Californian populations of the coral Balanophyllia elegans from distinct upwelling regimes, and test their physiological and transcriptomic responses to experimental seawater acidification. We measured respiration rates, protein and lipid content, and gene expression in corals from both populations exposed to pH levels of 7.8 and 7.4 for 29 days. Corals from the population that experiences lower pH due to high upwelling, maintained the same respiration rate throughout the exposure. In contrast, corals from the low upwelling site had reduced respiration rates, protein content, and lipid‐class content at low pH exposure, suggesting they have depleted their energy reserves. Using RNA‐Seq, we found that corals from the high upwelling site upregulated genes involved in calcium ion binding and ion transport, most likely related to pH homeostasis and calcification. In contrast, corals from the low upwelling site downregulated stress response genes at low pH exposure. Divergent population responses to low pH observed in B. elegans highlight the importance of multi‐population studies for predicting a species' response to future OA.
title Seawater carbonate chemistry and respiration rates, protein and lipid content of Balanophyllia elegans
topic Alkalinity, total; Animalia; Aragonite saturation state; Balanophyllia elegans; Benthic animals; Benthos; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2calc; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cnidaria; Coast and continental shelf; Dry mass; Event label; EXP; Experiment; Experiment duration; Fatty acids, total; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Globigerinatheka cf. index; Goleta; Identification; Incubation duration; Laboratory experiment; Lipids, total; Location; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, total scale; Phospholipid fatty acids; Point_Arena_Cove; Potentiometric; Potentiometric titration; Proteins, total; Registration number of species; Respiration; Respiration rate, oxygen; Salinity; Single species; Species; Sterols; Temperate; Temperature, water; Treatment; Triacylglycerols, per dry mass; Type; Uniform resource locator/link to reference
url https://doi.org/10.1594/PANGAEA.920209