Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019
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PANGAEA
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| Auteurs principaux: | , , , , , , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2020
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| _version_ | 1867170101756690432 |
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| author | Käse, Laura Kraberg, Alexandra Claudia Metfies, Katja Neuhaus, Stefan Sprong, Pim Fuchs, Bernhard M Boersma, Maarten Wiltshire, Karen Helen |
| author_facet | Käse, Laura Kraberg, Alexandra Claudia Metfies, Katja Neuhaus, Stefan Sprong, Pim Fuchs, Bernhard M Boersma, Maarten Wiltshire, Karen Helen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_921026 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019 Käse, Laura Kraberg, Alexandra Claudia Metfies, Katja Neuhaus, Stefan Sprong, Pim Fuchs, Bernhard M Boersma, Maarten Wiltshire, Karen Helen Binary Object; DNA barcoding; eukaryotic plankton; German Bight, North Sea; HelgolandRoads_site; Kabeltonne; OTU; Phytoplankton; protists; Zooplankton Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database. |
| title | Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019 |
| topic | Binary Object; DNA barcoding; eukaryotic plankton; German Bight, North Sea; HelgolandRoads_site; Kabeltonne; OTU; Phytoplankton; protists; Zooplankton |
| url | https://doi.org/10.1594/PANGAEA.921026 |