Thalassiosira rotula culture experiments for the assessment of phytoplankton stable carbon isotopic composition as a tool to monitor anthropogenic CO2 submarine leakages
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2020
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| author | Relitti, Federica Ogrinc, Nives Giani, Michele Cerino, Federica Smodlaka Tanković, Mirta Baričević, Ana Urbini, Lidia Krajnc, Bor Celussi, Mauro Kralj, Martina De Vittor, Cinzia |
| author_facet | Relitti, Federica Ogrinc, Nives Giani, Michele Cerino, Federica Smodlaka Tanković, Mirta Baričević, Ana Urbini, Lidia Krajnc, Bor Celussi, Mauro Kralj, Martina De Vittor, Cinzia |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Three culture experiment on the diatom Thalassiosira rotula were conducted within two 2L-photobioreactors under controlled conditions of light and temperature (20 ± 0.02 °C). During each experiment, diatoms were simultaneously grown in the two bioreactors filled respectively with different media. In one of the two bioreactor microalgae were grown in a natural seawater (NAT) medium, whereas in the second one an artificial seawater medium (ASW) was used. ASW medium was artificially prepared, to reach a modified isotopic composition of DIC using an industrial compressed CO2, and at each experiment a different initial DIC concentration was tested to verify if carbon availability would affect phytoplankton algal metabolism. For NAT medium, a sterile-filtered Adriatic Sea water was used and a natural DIC concentration used. During the experiments, the evolution of T. rotula abundance was investigated, together with the variation of the isotopic composition of dissolved inorganic carbon, phytoplankton organic carbon (as particulate organic carbon) and prokaryotes organic carbon (as the fraction < 10 µm of particulate organic carbon). Carbonate system parameters (in situ pHNBS, total alkalinity, DIC and dissolved CO2 concentration), nutrient (dissolved inorganic nitrogen and phosphate) concentration and prokaryotes abundance were also analysed during diatom cultivation. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_925185 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Thalassiosira rotula culture experiments for the assessment of phytoplankton stable carbon isotopic composition as a tool to monitor anthropogenic CO2 submarine leakages Relitti, Federica Ogrinc, Nives Giani, Michele Cerino, Federica Smodlaka Tanković, Mirta Baričević, Ana Urbini, Lidia Krajnc, Bor Celussi, Mauro Kralj, Martina De Vittor, Cinzia 13C carbon fixation; 2L-photobioreactors; Alkalinity, total; Autoanalyzer-Colorimeter; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon capture and storage; CCS; CO2 leakage; Code; Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module; culture experiment; diatoms; Electrochemical probe; Experiment; Flow-cytometer; IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples; Light microscope; Medium; Nitrogen, inorganic, dissolved; pH, NBS scale; Phosphate; Phytoplankton; Phytoplankton, δ13C, particulate organic carbon; Potentiometric open-cell titration; Prokaryotes; Prokaryotes, δ13C, particulate organic carbon; Thalassiosira rotula; Thalassiosira rotula, standard deviation; Time in hours; δ13C, dissolved inorganic carbon Three culture experiment on the diatom Thalassiosira rotula were conducted within two 2L-photobioreactors under controlled conditions of light and temperature (20 ± 0.02 °C). During each experiment, diatoms were simultaneously grown in the two bioreactors filled respectively with different media. In one of the two bioreactor microalgae were grown in a natural seawater (NAT) medium, whereas in the second one an artificial seawater medium (ASW) was used. ASW medium was artificially prepared, to reach a modified isotopic composition of DIC using an industrial compressed CO2, and at each experiment a different initial DIC concentration was tested to verify if carbon availability would affect phytoplankton algal metabolism. For NAT medium, a sterile-filtered Adriatic Sea water was used and a natural DIC concentration used. During the experiments, the evolution of T. rotula abundance was investigated, together with the variation of the isotopic composition of dissolved inorganic carbon, phytoplankton organic carbon (as particulate organic carbon) and prokaryotes organic carbon (as the fraction < 10 µm of particulate organic carbon). Carbonate system parameters (in situ pHNBS, total alkalinity, DIC and dissolved CO2 concentration), nutrient (dissolved inorganic nitrogen and phosphate) concentration and prokaryotes abundance were also analysed during diatom cultivation. |
| title | Thalassiosira rotula culture experiments for the assessment of phytoplankton stable carbon isotopic composition as a tool to monitor anthropogenic CO2 submarine leakages |
| topic | 13C carbon fixation; 2L-photobioreactors; Alkalinity, total; Autoanalyzer-Colorimeter; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon capture and storage; CCS; CO2 leakage; Code; Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module; culture experiment; diatoms; Electrochemical probe; Experiment; Flow-cytometer; IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples; Light microscope; Medium; Nitrogen, inorganic, dissolved; pH, NBS scale; Phosphate; Phytoplankton; Phytoplankton, δ13C, particulate organic carbon; Potentiometric open-cell titration; Prokaryotes; Prokaryotes, δ13C, particulate organic carbon; Thalassiosira rotula; Thalassiosira rotula, standard deviation; Time in hours; δ13C, dissolved inorganic carbon |
| url | https://doi.org/10.1594/PANGAEA.925185 |