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| Main Authors: | , , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2020
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| Online Access: | https://doi.org/10.1594/PANGAEA.925816 |
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| _version_ | 1867171023807315968 |
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| author | Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele |
| author_facet | Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The ongoing increase of CO2 in the atmosphere is inducing a progressive lowering of marine water pH that is predicted to decrease to 7.8 by the end of this century. In marine environment, physical perturbation may affect reproduction, which is crucial for species' survival and strictly depends on gamete quality. The effects of seawater acidification (SWAc) on gamete quality of broadcast spawning marine invertebrates result largely from experiments of gamete exposure while the SWAc impact in response to adult exposure is poorly investigated. Performing microcosm and in field experiments at a naturally acidified site, we investigated the effects of adult SWAc exposure on sperm quality parameters underlying fertilization in Mytilus galloprovincialis. These animals were exposed to pH 7.8 over 21 days and collected at different times to analyze sperm parameters as concentration, motility, viability, morphology, oxidative status, intra- and extra-cellular pH and mitochondrial membrane potential. Results obtained in the two experimental approaches were slightly different. Under field conditions, we found an increase in total sperm motility and mitochondrial membrane potential on days 7 and 14 from the start of SWAc exposure whereas, in microcosm, SWAc group showed an increase of total motility on day 14. In addition, sperm morphology and intracellular pH were affected in both experimental approaches; whereas oxidative stress was detected only in spermatozoa collected from mussels under natural SWAc. The overall analysis suggests that, in mussels, SWAc toxic mechanism in spermatozoa does not involve oxidative stress. This study represents the first report on mussel sperm quality impairment after adult SWAc exposure, which may affect fertilization success with negative ecological and economic consequences; it also indicates that, although naturally acidified areas represent ideal natural laboratories for investigating the impact of ocean acidification, microcosm experiments are necessary for examining action mechanisms. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_925816 |
| institution | PANGAEA |
| language | en |
| publishDate | 2020 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis Gallo, Alessandra Esposito, Maria Consiglia Cuccaro, Alessia Buia, Maria-Cristina Tarallo, Andrea Monfrecola, Vincenzo Tosti, Elisabetta Boni, Raffaele Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Coast and continental shelf; EXP; Experiment; Experiment day; Field experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Laboratory experiment; Lipid peroxidation; Lipid peroxidation, standard deviation; Mediterranean Sea; Mitochondrial membrane potential; Mitochondrial membrane potential, standard deviation; Mollusca; Mytilus galloprovincialis; Naples_OA; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, intracellular; pH, intracellular, standard deviation; pH, seminal; pH, seminal, standard deviation; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Reproduction; Salinity; Salinity, standard deviation; Single species; Species; Sperm concentration; Sperm concentration, standard deviation; Sperm motility; Sperm motility, standard deviation; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference; Viability; Viability, standard deviation The ongoing increase of CO2 in the atmosphere is inducing a progressive lowering of marine water pH that is predicted to decrease to 7.8 by the end of this century. In marine environment, physical perturbation may affect reproduction, which is crucial for species' survival and strictly depends on gamete quality. The effects of seawater acidification (SWAc) on gamete quality of broadcast spawning marine invertebrates result largely from experiments of gamete exposure while the SWAc impact in response to adult exposure is poorly investigated. Performing microcosm and in field experiments at a naturally acidified site, we investigated the effects of adult SWAc exposure on sperm quality parameters underlying fertilization in Mytilus galloprovincialis. These animals were exposed to pH 7.8 over 21 days and collected at different times to analyze sperm parameters as concentration, motility, viability, morphology, oxidative status, intra- and extra-cellular pH and mitochondrial membrane potential. Results obtained in the two experimental approaches were slightly different. Under field conditions, we found an increase in total sperm motility and mitochondrial membrane potential on days 7 and 14 from the start of SWAc exposure whereas, in microcosm, SWAc group showed an increase of total motility on day 14. In addition, sperm morphology and intracellular pH were affected in both experimental approaches; whereas oxidative stress was detected only in spermatozoa collected from mussels under natural SWAc. The overall analysis suggests that, in mussels, SWAc toxic mechanism in spermatozoa does not involve oxidative stress. This study represents the first report on mussel sperm quality impairment after adult SWAc exposure, which may affect fertilization success with negative ecological and economic consequences; it also indicates that, although naturally acidified areas represent ideal natural laboratories for investigating the impact of ocean acidification, microcosm experiments are necessary for examining action mechanisms. |
| title | Seawater carbonate chemistry and sperm physiology in Mytilus galloprovincialis |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Coast and continental shelf; EXP; Experiment; Experiment day; Field experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Laboratory experiment; Lipid peroxidation; Lipid peroxidation, standard deviation; Mediterranean Sea; Mitochondrial membrane potential; Mitochondrial membrane potential, standard deviation; Mollusca; Mytilus galloprovincialis; Naples_OA; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, intracellular; pH, intracellular, standard deviation; pH, seminal; pH, seminal, standard deviation; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Reactive oxygen species production; Reactive oxygen species production, standard deviation; Registration number of species; Reproduction; Salinity; Salinity, standard deviation; Single species; Species; Sperm concentration; Sperm concentration, standard deviation; Sperm motility; Sperm motility, standard deviation; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type; Uniform resource locator/link to reference; Viability; Viability, standard deviation |
| url | https://doi.org/10.1594/PANGAEA.925816 |