Genetic support for the morphological identification of larvae of Myctophidae, Gonostomatidae, Sternoptychidae and Phosichthyidae (Pisces) from the western Mediterranean
Fuente:
PANGAEA
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Dataset Open Access |
| Sprache: | en |
| Veröffentlicht: |
PANGAEA
2021
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1867169144256856064 |
|---|---|
| author | Bernal, Ainhoa Viñas, Jordi Olivar, M Pilar |
| author_facet | Bernal, Ainhoa Viñas, Jordi Olivar, M Pilar |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Mesopelagic fishes experience an extreme body transformation from larvae to adults. DNA barcoding has been useful in establishing phylogenetic relationships among mesopelagic and other deep-sea fishes, however no molecular evidence to confirm the identity of their larvae was available so far. Molecular analyses were conducted to identify the larvae and adults of 18 species of the most abundant and frequent mesopelagic fishes in the western Mediterranean, across 14 genera. For myctophids, 7 of 9 species of the subfamily Lampanyctinae and all species from Myctophinae (6) were analysed. At least 565 nucleotides of the mtDNA CO1 region were sequenced. The 12S rRNA region was selected for C. braueri and C. pygmaea (372 nucleotides). Pairwise nucleotide distances were notably higher inter- than intraspecifically allowing to discern between congeners such as Cyclothone braueri and C. pygmaea, Hygophum benoiti and H. hygomii, Lampanyctus crocodilus and L. pusillus, and Notoscopelus bolini and N. elongatus. The good fit between larvae and adult sequences for each species proved the accuracy of earlier larval descriptions based on morphology and pigmentation characters. A list of the genetic markers used to identify each species with their accession numbers in Genbank is provided here along with their developmental stage, sampling location and depth. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_928703 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Genetic support for the morphological identification of larvae of Myctophidae, Gonostomatidae, Sternoptychidae and Phosichthyidae (Pisces) from the western Mediterranean Bernal, Ainhoa Viñas, Jordi Olivar, M Pilar 12S ribosomal ribonucleic acid; ABI Prism 310 Genetic Analyzer, Applied Biosystems; Accession number, genetics; Balearic Islands, western Mediterranean Sea; cytochrome c oxidase subunit 1; Date/time end; Date/time start; DEPTH, water; DNA-barcoding; Estructura y dinámica del ecosistema bentopelágico de talud en dos zonas oligotróficas del Mediterráneo: una aproximación multidisciplinar y a distintas escalas temporales en las Islas Baleares; Event label; Family; Gene name; IDEADOS; IDEADOS_0710; IDEADOS_2010_07-1; IDEADOS_2010_08-1; IDEADOS_2010_09-1; IDEADOS_2010_12-1; IDEADOS_2010_14-1; IDEADOS_2010_15-1; IDEADOS_2010_17-1; IDEADOS_2010_IKMT_08; IDEADOS_2010_IKMT_09_F; IDEADOS_2010_IKMT_09_SUP; IDEADOS_2010_IKMT_17; IDEADOS_2010_IKMT_17_400; IDEADOS_2010_IKMT_18_F; IDEADOS_2010_IKMT_19_INT; IDEADOS_2010_IKMT_19_SUP; IDEADOS_2010_IKMT_20; IDEADOS_2010_IKMT_21_SUP; IDEADOS_2010_IKMT_22_SUP; IDEADOS_2010_IKMT_23_F; IDEADOS_2010_IKMT_23_SUP; IDEADOS_2010_IKMT_26_F; IDEADOS_2010_MOC_02; IDEADOS_2010_MOC_06; IDEADOS_2010_MOC_10; IDEADOS_2010_MOC_12; IDEADOS_2010_MOC_14; IDEADOS_2010_MOC_15; IDEADOS_2010_MOC_18-900; IDEADOS_2010_MOC_19-500; IDEADOS_2010_MOC_19-900; IDEADOS_2010_MOC_20; IDEADOS_2010_MOC_21; IDEADOS_2010_MOC_22; IDEADOS_2010_WP2_03_F; IDEADOS-10; Identification; IKMT; Isaacs-Kidd Midwater Trawl; larval identification; Latitude of event; Latitude of event 2; Life stage; Longitude of event; Longitude of event 2; mesopelagic fish; Midwater trawl, Pelagica 112 m²; MOC1; MOCNESS opening/closing plankton net 1 sqm; Pelagica 112; Sarmiento de Gamboa; Sequence length; Species; Station label; SUMMER; Sustainable Management of Mesopelagic Resources; Western Mediterranean Sea; WP2; WP-2 towed closing plankton net Mesopelagic fishes experience an extreme body transformation from larvae to adults. DNA barcoding has been useful in establishing phylogenetic relationships among mesopelagic and other deep-sea fishes, however no molecular evidence to confirm the identity of their larvae was available so far. Molecular analyses were conducted to identify the larvae and adults of 18 species of the most abundant and frequent mesopelagic fishes in the western Mediterranean, across 14 genera. For myctophids, 7 of 9 species of the subfamily Lampanyctinae and all species from Myctophinae (6) were analysed. At least 565 nucleotides of the mtDNA CO1 region were sequenced. The 12S rRNA region was selected for C. braueri and C. pygmaea (372 nucleotides). Pairwise nucleotide distances were notably higher inter- than intraspecifically allowing to discern between congeners such as Cyclothone braueri and C. pygmaea, Hygophum benoiti and H. hygomii, Lampanyctus crocodilus and L. pusillus, and Notoscopelus bolini and N. elongatus. The good fit between larvae and adult sequences for each species proved the accuracy of earlier larval descriptions based on morphology and pigmentation characters. A list of the genetic markers used to identify each species with their accession numbers in Genbank is provided here along with their developmental stage, sampling location and depth. |
| title | Genetic support for the morphological identification of larvae of Myctophidae, Gonostomatidae, Sternoptychidae and Phosichthyidae (Pisces) from the western Mediterranean |
| topic | 12S ribosomal ribonucleic acid; ABI Prism 310 Genetic Analyzer, Applied Biosystems; Accession number, genetics; Balearic Islands, western Mediterranean Sea; cytochrome c oxidase subunit 1; Date/time end; Date/time start; DEPTH, water; DNA-barcoding; Estructura y dinámica del ecosistema bentopelágico de talud en dos zonas oligotróficas del Mediterráneo: una aproximación multidisciplinar y a distintas escalas temporales en las Islas Baleares; Event label; Family; Gene name; IDEADOS; IDEADOS_0710; IDEADOS_2010_07-1; IDEADOS_2010_08-1; IDEADOS_2010_09-1; IDEADOS_2010_12-1; IDEADOS_2010_14-1; IDEADOS_2010_15-1; IDEADOS_2010_17-1; IDEADOS_2010_IKMT_08; IDEADOS_2010_IKMT_09_F; IDEADOS_2010_IKMT_09_SUP; IDEADOS_2010_IKMT_17; IDEADOS_2010_IKMT_17_400; IDEADOS_2010_IKMT_18_F; IDEADOS_2010_IKMT_19_INT; IDEADOS_2010_IKMT_19_SUP; IDEADOS_2010_IKMT_20; IDEADOS_2010_IKMT_21_SUP; IDEADOS_2010_IKMT_22_SUP; IDEADOS_2010_IKMT_23_F; IDEADOS_2010_IKMT_23_SUP; IDEADOS_2010_IKMT_26_F; IDEADOS_2010_MOC_02; IDEADOS_2010_MOC_06; IDEADOS_2010_MOC_10; IDEADOS_2010_MOC_12; IDEADOS_2010_MOC_14; IDEADOS_2010_MOC_15; IDEADOS_2010_MOC_18-900; IDEADOS_2010_MOC_19-500; IDEADOS_2010_MOC_19-900; IDEADOS_2010_MOC_20; IDEADOS_2010_MOC_21; IDEADOS_2010_MOC_22; IDEADOS_2010_WP2_03_F; IDEADOS-10; Identification; IKMT; Isaacs-Kidd Midwater Trawl; larval identification; Latitude of event; Latitude of event 2; Life stage; Longitude of event; Longitude of event 2; mesopelagic fish; Midwater trawl, Pelagica 112 m²; MOC1; MOCNESS opening/closing plankton net 1 sqm; Pelagica 112; Sarmiento de Gamboa; Sequence length; Species; Station label; SUMMER; Sustainable Management of Mesopelagic Resources; Western Mediterranean Sea; WP2; WP-2 towed closing plankton net |
| url | https://doi.org/10.1594/PANGAEA.928703 |