Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2021
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| _version_ | 1867170105198116864 |
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| author | Qu, Liming Campbell, Douglas A Gao, Kunshan |
| author_facet | Qu, Liming Campbell, Douglas A Gao, Kunshan |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_928761 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom Qu, Liming Campbell, Douglas A Gao, Kunshan Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carotenoids, standard deviation; Carotenoids/Chlorophyll a ratio; Carotenoids/Chlorophyll a ratio, standard deviation; Carotenoids per cell; Cell size; Cell size, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Effective quantum yield; Effective quantum yield, standard deviation; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Electron transport rate efficiency; Electron transport rate efficiency, standard deviation; Extracellular carbonic anhydrase activity, per cell; Extracellular carbonic anhydrase activity, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Laboratory experiment; Laboratory strains; Light; Light saturation point; Light saturation point, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Maximum quantum yield of photosystem II; Maximum quantum yield of photosystem II, standard deviation; Net photosynthesis rate, oxygen, per cell; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Oxygen evolution, daytime; Oxygen evolution, daytime, standard deviation; Oxygen evolution per cell, daytime; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Phytoplankton; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Registration number of species; Respiration; Respiration rate, oxygen, per cell; Respiration rate, oxygen, per chlorophyll a; Respiration rate, oxygen, standard deviation; Salinity; Single species; Species; Temperature, water; Thalassiosira weissflogii; Time in days; Treatment; Type; Uniform resource locator/link to reference Diatom responses to ocean acidification have been documented with variable and controversial results. We grew the coastal diatom Thalassiosira weissflogii under 410 (LC, pH 8.13) vs 1000 μatm (HC, pH 7.83) pCO2 and at different levels of light (80, 140, 220 μmol photons/m**2/s), and found that light level alters physiological responses to OA. CO2 concentrating mechanisms (CCMs) were down-regulated in the HC-grown cells across all the light levels, as reflected by lowered activity of the periplasmic carbonic anhydrase and decreased photosynthetic affinity for CO2 or dissolved inorganic carbon. The specific growth rate was, however, enhanced significantly by 9.2% only at the limiting low light level. These results indicate that rather than CO2 “fertilization”, the energy saved from down-regulation of CCMs promoted the growth rate of the diatom when light availability is low, in parallel with enhanced respiration under OA to cope with the acidic stress by providing extra energy. |
| title | Seawater carbonate chemistry and CO2 acquisition efficiency and mitochondrial respiration in a coastal diatom |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carotenoids, standard deviation; Carotenoids/Chlorophyll a ratio; Carotenoids/Chlorophyll a ratio, standard deviation; Carotenoids per cell; Cell size; Cell size, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Effective quantum yield; Effective quantum yield, standard deviation; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Electron transport rate efficiency; Electron transport rate efficiency, standard deviation; Extracellular carbonic anhydrase activity, per cell; Extracellular carbonic anhydrase activity, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Laboratory experiment; Laboratory strains; Light; Light saturation point; Light saturation point, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Maximum quantum yield of photosystem II; Maximum quantum yield of photosystem II, standard deviation; Net photosynthesis rate, oxygen, per cell; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Ochrophyta; Oxygen evolution, daytime; Oxygen evolution, daytime, standard deviation; Oxygen evolution per cell, daytime; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Phytoplankton; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Registration number of species; Respiration; Respiration rate, oxygen, per cell; Respiration rate, oxygen, per chlorophyll a; Respiration rate, oxygen, standard deviation; Salinity; Single species; Species; Temperature, water; Thalassiosira weissflogii; Time in days; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.928761 |