Seawater carbonate chemistry and sperm motility of Tegillarca granosavia
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2021
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| _version_ | 1867171873878441984 |
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| author | Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu |
| author_facet | Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_929195 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and sperm motility of Tegillarca granosavia Shi, Wei Han, Yu Huang, Xianke Hu, Lihua Chai, Xueliang Liu, Guangxu 6-phosphofructokinase activity, unit per protein mass; Adenosine triphosphate, per unit protein; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Average path velocity; Average path velocity, standard error; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calcium-ATPase activity; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Curvilinear velocity; Curvilinear velocity, standard error; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; Potentiometric; Potentiometric titration; Pyruvate kinase activity, unit per protein mass; Registration number of species; Salinity; Salinity, standard error; Single species; Species; Straight line velocity; Straight line velocity, standard error; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference; Yueqing_Bay_OA Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation. |
| title | Seawater carbonate chemistry and sperm motility of Tegillarca granosavia |
| topic | 6-phosphofructokinase activity, unit per protein mass; Adenosine triphosphate, per unit protein; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Average path velocity; Average path velocity, standard error; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calcium-ATPase activity; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Curvilinear velocity; Curvilinear velocity, standard error; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH, NBS scale; pH, standard error; pH, total scale; Potentiometric; Potentiometric titration; Pyruvate kinase activity, unit per protein mass; Registration number of species; Salinity; Salinity, standard error; Single species; Species; Straight line velocity; Straight line velocity, standard error; Tegillarca granosa; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference; Yueqing_Bay_OA |
| url | https://doi.org/10.1594/PANGAEA.929195 |